Core Facility

Fan Liu

Mass Spectrometry

Fan Liu

The mass spectrometry facility hosts state-of-the-art infrastructure for protein characterization. This includes high-end MS technologies for protein identification, quantification, post-translational modification and interaction analysis.


Profile

PROTEOMICS AND MASS SPECTROMETRY
Proteins are functional cornerstones of the cell and alterations in protein expression, post-translational modification (PTM), structure and interactions are linked to every physiological and pathological process. The aim of the MS facility is to provide cutting-edge MS technologies for protein characterization in all aformentioned perspectives.
We host a range of state-of-the-art mass spectrometers, including Exploris 480, Orbitrap Astral, Orbitrap Fusion Lumos ETD and Orbitrap Fusion ETD (Thermo Fisher Scientific), coupled to ultra-performance liquid chromatography (UPLC) systems.
Furthermore, we are also equipped with standalone high-performance liquid chromatography (HPLC) system for peptide separation and skilled in advanced sample preparing procedures, assuring the delivery of reliable and reproducible results to our customers and collaborators.

Services

The MS core facility offers a wide range of services related to protein characterization. This includes but not limited to:

  • protein identification from gels or in solution.
  • affinity purification MS to identify protein interaction partners.
  • global proteome quantification using well-established quantitative proteomics approaches, including label-free, SILAC and TMT strategies.
  • protein post-translational modification (PTM) analysis.
  • protein structure and interaction profiling by cross-linking mass spectrometry.
  • protein and peptide separation by liquid chromatography, including basic reversed phase separation, size exclusion chromatography and ion exchange chromatography.

Sample Submission

First time user: please contact us to discuss your project.

Regular users: please fill in the sample submission form to submit your samples.

Method development

In addition to routine services, the MS core facility also actively involves in method development tailored to specific collaborative projects. Our latest advancements focus on 1) establishing proteome-wide cross-linking mass spectrometry pipeline (in collaboration with Thermo Fisher Scientific) and 2) developing novel data acquisition strategies for labile protein post-translational modifications, in particular pyrophosphorylation (in collaboration with Prof. Dorothea Fiedler) as well as Cys, Lys and His phosphorylation (in collaboration with Prof. Christian Hackenberger). For more details, please refer to our publications.

Contact

Prof. Dr. Fan Liu

Head, Fan Liu (SI) Group,
Head Mass Spectrometry


Research Section

Structural Biology

Publications via ORCID

of 5

PROTEOME-SCALE RECOMBINANT STANDARDS AND A ROBUST HIGH-SPEED SEARCH ENGINE TO ADVANCE CROSS-LINKING MS-BASED INTERACTOMICS

  • Milan Avila Clasen; Max Ruwolt; Louise U. Kurt; Fabio C Gozzo; Shuai Wang; Tao Chen; Paulo C Carvalho; Diogo Borges Lima; Fan Liu
read online

Real-Time Library Search Increases Cross-Link Identification Depth across All Levels of Sample Complexity

  • Max Ruwolt; Yi He; Diogo Borges Lima; William Barshop; Johannes Broichhagen; Romain Huguet; Rosa Viner; Fan Liu

Analytical Chemistry 2023

read online

The potential of cross-linking mass spectrometry in the development of protein–protein interaction modulators

  • Max Ruwolt; Ilaria Piazza; Fan Liu

Current Opinion in Structural Biology 2023

read online

An antagonism between Spinophilin and Syd-1 operates upstream of memory-promoting presynaptic long-term plasticity

  • Niraja Ramesh; Marc Escher; Oriane Turrel; Janine Lützkendorf; Tanja Matkovic; Fan Liu; Stephan Sigrist

eLife 2023

read online

CaMKII autophosphorylation can occur between holoenzymes without subunit exchange

  • Iva Lučić; Léonie Héluin; Pin-Lian Jiang; Alejandro G Castro Scalise; Cong Wang; Andreas Franz; Florian Heyd; Markus Wahl; Fan Liu; Andrew Plested

eLife 2023

read online

Spatially resolved protein map of intact human cytomegalovirus virions

  • Boris Bogdanow; Iris Gruska; Lars Mühlberg; Jonas Protze; Svea Hohensee; Barbara Vetter; Jens B. Bosse; Martin Lehmann; Mohsen Sadeghi; Lüder Wiebusch; Fan Liu

Nature Microbiology 2023

read online

Structural Host-Virus Interactome Profiling of Intact Infected Cells

  • Boris Bogdanow; Lars Mühlberg; Iris Gruska; Barbara Vetter; Julia Ruta; Arne Elofsson; Lüder Wiebusch; Fan Liu
read online
of 7
of 4
of 5