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PROTEOMICS AND MASS SPECTROMETRY
Proteins are functional cornerstones of the cell and alterations in protein expression, post-translational modification (PTM), structure and interactions are linked to every physiological and pathological process. The aim of the MS facility is to provide cutting-edge MS technologies for protein characterization in all aformentioned perspectives.
We host a range of state-of-the-art mass spectrometers, including Exploris 480, Orbitrap Astral, Orbitrap Fusion Lumos ETD and Orbitrap Fusion ETD (Thermo Fisher Scientific), coupled to ultra-performance liquid chromatography (UPLC) systems.
Furthermore, we are also equipped with standalone high-performance liquid chromatography (HPLC) system for peptide separation and skilled in advanced sample preparing procedures, assuring the delivery of reliable and reproducible results to our customers and collaborators.
The MS core facility offers a wide range of services related to protein characterization. This includes but not limited to:
First time user: please contact us to discuss your project.
Regular users: please fill in the sample submission form to submit your samples.
In addition to routine services, the MS core facility also actively involves in method development tailored to specific collaborative projects. Our latest advancements focus on 1) establishing proteome-wide cross-linking mass spectrometry pipeline (in collaboration with Thermo Fisher Scientific) and 2) developing novel data acquisition strategies for labile protein post-translational modifications, in particular pyrophosphorylation (in collaboration with Prof. Dorothea Fiedler) as well as Cys, Lys and His phosphorylation (in collaboration with Prof. Christian Hackenberger). For more details, please refer to our publications.
Thermo Orbitrap Fusion Lumos ETD, coupled to UltiMate 3000 RSLC nano LC system, coupled with Trajan LEAP HDX Platform
Thermo Orbitrap Fusion ETD, coupled to UltiMate 3000 RSLC nano LC system
picture of instrumentation
picture of instrumentation
Agilent 1260 Infinity II UPLC
Thermo Orbitrap Astral Mass Spectrometer, coupled to Vanquish Neo UHPLC System
Vanquish Flex UHPLC System
Bravo Automated Liquid Handling Platform
Thermo Orbitrap Exploris 480, coupled to Vanquish Neo UHPLC System
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