PROTEOMICS AND MASS SPECTROMETRY
Proteins are functional cornerstones of the cell and alterations in protein expression, post-translational modification (PTM), structure and interactions are linked to every physiological and pathological process. The aim of the MS facility is to provide cutting-edge MS technologies for protein characterization in all aformentioned perspectives.
We host a range of state-of-the-art mass spectrometers, including Orbitrap Fusion Lumos ETD, Orbitrap Fusion ETD and Orbitrap Elite (Thermo Fisher Scientific), coupled to ultra-performance liquid chromatography (UPLC) systems.
Furthermore, we are also equipped with standalone high-performance liquid chromatography (HPLC) system for peptide separation and skilled in advanced sample preparing procedures, assuring the delivery of reliable and reproducible results to our customers and collaborators.
The MS core facility offers a wide range of services related to protein characterization. This includes but not limited to:
In addition to routine services, the MS core facility also actively involves in method development tailored to specific collaborative projects. Our latest advancements focus on 1) establishing proteome-wide cross-linking mass spectrometry pipeline (in collaboration with Thermo Fisher Scientific) and 2) developing novel data acquisition strategies for labile protein post-translational modifications, in particular pyrophosphorylation (in collaboration with Prof. Dorothea Fiedler) as well as Cys, Lys and His phosphorylation (in collaboration with Prof. Christian Hackenberger). For more details, please refer to our publications.
Research SectionStructural Biology
XlinkCyNET: A Cytoscape Application for Visualization of Protein Interaction Networks Based on Cross-Linking Mass Spectrometry Identifications
Journal of Proteome Research 2021read online
Novel amyloid-beta pathology C. elegans model reveals distinct neurons as seeds of pathogenicity
Progress in Neurobiology 2021read online
eIF5A hypusination, boosted by dietary spermidine, protects from premature brain aging and mitochondrial dysfunction
Cell Reports 2021read online
Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions
Science Advances 2020read online
MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome–nascent chain complex
EMBO reports 2020read online
RIM-binding protein couples synaptic vesicle recruitment to release sites
Journal of Cell Biology 2020read online
Expanding the depth and sensitivity of cross-link identification by differential ion mobility using FAIMS
Analytical Chemistry 2020read online
Deciphering the Interplay among Multisite Phosphorylation, Interaction Dynamics, and Conformational Transitions in a Tripartite Protein System.
MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis
Journal of Biological Chemistry 2016read online
Hybrid mass spectrometry approaches in glycoprotein analysis and their usage in scoring biosimilarity
Nature Communications 2016read online
Interrogating the architecture of protein assemblies and protein interaction networks by cross-linking mass spectrometry.
Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry.
The Soluble Periplasmic Domains of Escherichia coli Cell Division Proteins FtsQ/FtsB/FtsL Form a Trimeric Complex with Submicromolar Affinity.
Benchmarking multiple fragmentation methods on an orbitrap fusion for top-down phospho-proteoform characterization.
Performing protein crosslinking using gas-phase cleavable chemical crosslinkers and liquid chromatography-tandem mass spectrometry.
Thermo Orbitrap Fusion Lumos ETD, coupled to UltiMate 3000 RSLC nano LC system
Thermo Orbitrap Fusion ETD, coupled to UltiMate 3000 RSLC nano LC system
Thermo Orbitrap Elite, coupled to UltiMate 3000 RSLC nano LC system, coupled with Trajan LEAP HDX Platform
picture of instrumentation
picture of instrumentation
Agilent 1260 Infinity II UPLC