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The mass spectrometry facility hosts state-of-the-art infrastructure for protein characterization. This includes high-end MS technologies for protein identification, quantification, post-translational modification and interaction analysis.
PROTEOMICS AND MASS SPECTROMETRY
Proteins are functional cornerstones of the cell and alterations in protein expression, post-translational modification (PTM), structure and interactions are linked to every physiological and pathological process. The aim of the MS facility is to provide cutting-edge MS technologies for protein characterization in all aformentioned perspectives.
We host a range of state-of-the-art mass spectrometers, including Orbitrap Fusion Lumos ETD, Orbitrap Fusion ETD and Orbitrap Elite (Thermo Fisher Scientific), coupled to ultra-performance liquid chromatography (UPLC) systems.
Furthermore, we are also equipped with standalone high-performance liquid chromatography (HPLC) system for peptide separation and skilled in advanced sample preparing procedures, assuring the delivery of reliable and reproducible results to our customers and collaborators.
The MS core facility offers a wide range of services related to protein characterization. This includes but not limited to:
First time user: please contact us to discuss your project.
Regular users: please fill in the sample submission form to submit your samples.
In addition to routine services, the MS core facility also actively involves in method development tailored to specific collaborative projects. Our latest advancements focus on 1) establishing proteome-wide cross-linking mass spectrometry pipeline (in collaboration with Thermo Fisher Scientific) and 2) developing novel data acquisition strategies for labile protein post-translational modifications, in particular pyrophosphorylation (in collaboration with Prof. Dorothea Fiedler) as well as Cys, Lys and His phosphorylation (in collaboration with Prof. Christian Hackenberger). For more details, please refer to our publications.
Thermo Orbitrap Fusion Lumos ETD, coupled to UltiMate 3000 RSLC nano LC system
Thermo Orbitrap Fusion ETD, coupled to UltiMate 3000 RSLC nano LC system
Thermo Orbitrap Elite, coupled to UltiMate 3000 RSLC nano LC system, coupled with Trajan LEAP HDX Platform
picture of instrumentation
picture of instrumentation
Agilent 1260 Infinity II UPLC
People
The Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) is part of the Forschungsverbund Berlin e.V. (FVB), which legally represents seven non-university research institutes - members of the Leibniz Association - in Berlin.
Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10,
13125 Berlin, Germany
