Mission

Open access for academic projects and SME´s towards experiments in high-throughput is our major aim. The idea behind is to support systematic drug screening and/or genome-wide functional interference studies (RNA-interference and CRISPR-CAS gene editing) to explore and validate novel drug targets for contribution towards disease development by using biological test systems reflecting health and dysregulated states. Beyond this, we also educate pupils, students and teachers about the scientific value provided by this applied combination of Chemistry and Biology methodology for a better understanding of the underlying molecular mechanisms of evolution of life. 

Our scientific focus is actually on the combination of Rudolf Virchows idea of Cell Pathology reflecting disease states (1848) and the idea of Anne Carpenter (Broad Institute) to apply fluorescent Cell Painting and artifiacial intelligence to recognize cell morphology patterns, which specifically mark cellular dysfunctions/diseases to unravel molecular mechanisms underlying disease development for novel diagnositic procedures and control of theraphy response.   

The Screening Unit serves for the Chemical Biology Platform of the FMP. This platform extends the collaborative support of academic projects and SME´s  through the Screening Unit ( HTS, Jens Peter von Kries) by Computational Simulations ( Han Sun) and Medicinal Chemistry (Marc Nazaré).

Within this platform the mission of the Screening Unit is to

  • provide well designed (World Drug Index derived druglike scaffolds) screening collections (70.000 cpds) including about 3.000 FDA approved drugs (SELLECK library), 1280 compounds with annotated activities (LOPAC library) and novel molecules synthesized and donated from academia (about 4.000 cpds). 30.000 cpds are either natural product derived synthetic molecules or purified from plants or bacteria provided by (Analyticon Discovery GmbH). For a detailed overview please follow this link.
  • provide additional hundred thousands compounds from EU-OPENSCREEN
  • identify bioactive small molecules as tools for modulation of biological functions for research termed Chemical Biology.
  • downscale test volumes ( low volume 384well plates: minimum 5 µl) to reduce costs for expensive reagents

More then 400 screening projects (Highlights) have been already supported in EU (MolDiaPaca, SFMET, AntiFlu), BMBF (Mtub, GoBio, NGFN...), DFG (FG-806) and TSB/IBB (Profit) funded work since 2004. Several projects are already either at the beginning or within clinical trials for patients suffering from cancer or rare disease like head stroke due to malformation of blood vessels.

The Unit served for academic and SME partners:

  • dnaAcos, Oslo (Norway)
  • Redoxis, Gothenburg (Sweden)
  • Jerini AG, Berlin (Germany).

For development of hardware:

  • Tecan Austria & Switzerland
  • Cellomics Inc. (Thermofisher)

Data documentation and analysis have been automated according to defined standard operating procedures and analysis pipelines. About 40.000 compounds can be screened (per pipetting workstation, total of 4) and analyzed in a single day (12 hrs) depending on the test system/detection method used. A complex set of reports containing heat maps, different statistical analysis routines, similarity clustering and inter screen analysis for identification of frequent hitters is provided.

Furthermore the Screening Unit tests novel detection systems, develops analysis and object identification routines, and in the case of positive outcomes integrates these into the daily screening service. For instance the usage of machine learning routines in collaboration with the ETH-Zürich for automated object identification in screening with automated microscopes. The latest technologies integrated are

  • label-free impedance measurement  (Excelligence, 96well/384well)
  • surface plasmon resonance             (Biacore T200)
  • high speed kinetic imaging               (FLIPR-Tetra)

(see "Technologies" for more detail).

Beside this service, the Screening Unit also manages a technology platform for genome-wide RNA-interference  in C. elegans, mouse and in human cell lines.

IMPORTANT NOTICE: As genome-wide RNA-interference may require large amounts of expensive liposome reagents to screen up to 800 MTP´s in 384well format, it is a very expensive approach, especially in combination with dual-luciferase reporters. Therefore you may wish to contact us to discuss alternative reporter systems and to estimate the required funding budgets.