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We thrive to understand the molecular mechanism underlying the interaction of the intestinal microbiome with the human host. We focus on bacteria that are overrepresented in the microbiome of colorectal cancer (CRC) patients and the interactions of bacterial adhesins with epithelial and immune cells of the intestinal system. Genomic sequencing, big data analysis, and substantial microbial studies in animal models identified important drivers of CRC on the microbial and cellular level, but the underlying mechanistic details are not known. Therefore, we structurally analyze complexes between commensal proteins and human receptors to understand the molecular background of their binding mechanisms.
We apply cryogenic electron microscopy (Cryo-EM) and single particle analysis to determine the structures of protein complexes that facilitate the host-microbiome interaction, which is a prerequisite for structure-based design of personalized anticancer compounds. In addition, we visualize molecular details of bacteria interacting with the host epithel via cryo-ET to understand their binding mechanisms in a cellular context. Finally, we attempt to identify yet undescribed bacterial adhesins that facilitate epithelial binding in a pathogenic context using genomic and biochemical screening systems.
People
current position: Postdoc at Institut Curie, Paris.
Sylvestre studied chemistry at the ENSC in Montpellier and obtained his PhD in 2016 in Organic Chemistry (Marie Curie ITN grant) in the group of Joseph Harrity at U Sheffield, UK, including a six-month secondment with Stefan Bräse at the KIT in Germany. He joined the group of Stephen Hanessian at U Montréal, worked at the ENS Paris, with Blaise Dumat, Laurence Grimaud and Maxime Vitale, before looking into bioconjugation with Patricia Busca at Université Paris Cité. In 2024, he joined the groups of JB and Daniel Roderer at FMP to work on the development of Cryo-CLEM probes supported by CZI.
current position: Cellular Imaging facility, FMP Berlin
If you are interested in a Master thesis position, please contact Daniel Roderer via email.
G. L. Marongiu*, U. Fink*, F. Schöpf, A. Oder, J. P. von Kries and Daniel Roderer. Structural basis for immune cell binding of Fusobacterium nucleatum via the trimeric autotransporter adhesin CbpF. PNAS, 122(15), 2025. doi: 10.1073/pnas.2418155122.
P. N. Ng’ang’a*, J. Folz*, S. Kucher*, D. Roderer*, Y. Xu, O. Sitsel, A. Belyy, D. Prumbaum, R. Kühnemuth, T. E. Assafa, M. Dong, C. A. M. Seidel, E. Bordignon and S. Raunser. Multistate kinetics of the syringe-like injection mechanism of Tc toxins. Science Advances 11 (1), 2025. doi: 10.1126/sciadv.adr2019.
S.K. Nagarajan, J. Weber, D. Roderer and J. Piontek. C. perfringens enterotoxin-claudin pore complex: Models for structure, mechanism of pore assembly and cation permeability. Computational and Structural Biotechnology Journal 27 (287-306), 2025. doi: 10.1016/j.csbj.2024.11.048.
A. Celik, F. Schöpf, C.E. Sieger, J.A.M. Morgan, S. Lampe, M. Ruwolt, F. Liu, C.P.R. Hackenberger, D. Roderer and D. Fiedler. Nucleoside diphosphate kinase A (NME1) catalyzes its own oligophosphorylation. Preprint at bioRxiv, 2024. doi: 10.1101/2024.07.29.605581.
F. Schöpf, G. L. Marongiu, K. Milaj, T. Sprink, J. Kikhney, A. Moter and D. Roderer. Structural basis of Fusobacterium nucleatum adhesin Fap2 interaction with receptors on cancer and immune cells. Preprint at bioRxiv, 2024. doi: 10.1101/2024.02.28.582045.
M. Shafaq-Zadah*, E. Dransart, C. Wunder, V. Chambon, C. A. Valades-Cruz, L. Leconte, N. Kumar Sarangi, J. Robinson, S. Bai, R. Regmi, A. Di Cicco, A. Hovasse, R. Bartels, U. Nilsson, S. Cianférani-Sanglier, H. Leffler, T. Keyes, D. Lévy, S. Raunser, D. Roderer*, L. Johannes*. Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate. Preprint at bioRxiv, 2023. doi: 10.1101/2023.10.27.564026.
This text and the images are published under the Creative Commons licence "CC BY-NC-ND 4.0 EN - Attribution-Non-Commercial-No Derivative Works 4.0 Germany". Author: Daniel Roderer for leibniz-fmp.de
You may share the text by mentioning the licence CC BY-NC-ND 4.0 DE and the author(s). Copyright information on images/graphics/videos can be found directly with the images and additionally in the appendix.