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We are interested in the molecular mechanism of host-microbiome interactions in the human intestinal system, which play important roles in onset and progression of diseases. We therefore apply Cryo-EM to solve the structures of protein complexes that facilitate epithelial adhesion of bacteria.
We thrive to understand the molecular mechanism underlying the interaction of the intestinal microbiome with the human host. We focus on bacteria that are overrepresented in the microbiome of colorectal cancer (CRC) patients and the interactions of bacterial adhesins with epithelial and immune cells of the intestinal system. Meta-Omics and substantial microbial studies in animal models identified important drivers of CRC on the microbial and cellular level, but the underlying mechanistic details are not known. To close this major gap of knowledge, we apply cryogenic electron microscopy (Cryo-EM) and single particle analysis to determine the structures of protein complexes that facilitate the host-microbiome interaction, a prerequisite for structure-based design of personalized anticancer compounds. To understand the context of how different adhesins act together in the cellular context, we apply cryo-ET to visualize molecular details of bacteria interacting with the host epithel and to integrate the in vitro structures into the in situ context.
With numerous biological interactions involving membrane proteins and a general shortcoming of the underlying mechanistic details, the Roderer lab strives to decipher mechanistic details of action of membrane proteins and glycan-protein interactions. These include bacterial toxins that recognize human claudins at tight junctions, rhomboid proteases and their substrate recognition mechanism, and α5β1 integrin whose numerous glycosylation sites interact with and oligomerize galectin-3 in a conformational state-specific manner.
People
current position: Postdoc at Institut Curie, Paris.
current position: Cellular Imaging facility, FMP Berlin
If you are interested in a Master thesis position, please contact Daniel Roderer via email.
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The Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) is part of the Forschungsverbund Berlin e.V. (FVB), which legally represents seven non-university research institutes - members of the Leibniz Association - in Berlin.
Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Rudower Chaussee 17
12489 Berlin, Germany
