Core FacilityMolecular Physiology & Cell Biology

Ralf Schülein

Cell Engineering

potrait ralf schuelein

The Cell Engineering Facility uses state of the art CRISPR/Cas techniques to generate gene knock-outs and knock-ins in target cells. Other applications of CRISPR/Cas may be possible following consultation.

Gene knock-out means that the function of a specific gene, and consequently that of its encoded protein, is disrupted by a specific mutation. Gene knock-in means that the specific target gene and its protein gain a new function because of an engineered mutation. By using the resulting CRISPR/Cas cell clones, the function of specific genes and proteins can be studied in detail within the cellular context.


General Information

After its publication in 2012, the CRISPR/Cas gene editing system revolutionized molecular biology within only a few years (CRISPR = clustered regularly interspaced short palindromic repeats; see also our Introduction page). The increase in publications using CRISPR/Cas is only comparable to what was seen in the eighties following the development of the polymerase chain reaction (PCR) or in the nineties following the introduction of the green fluorescent protein (GFP). The Cell Engineering Facility of the FMP will make gene knock-outs and knock-ins in various target cells using state-of-the art CRISPR/Cas techniques. Other applications may be possible following consultation. Cell clones will be characterized by DNA sequencing and the genotype will be analyzed to confirm modification of both alleles. Western blotting will be done in case you could provide a specific antibody.
An overview of the methodology we use can be found on the “Methods” page. If you are already familiar with CRISPR/Cas, you may immediately proceed to the “Order” page. Depending on the amount of simultaneous orders, clones should be ready within 8-12 weeks depending on cell type. If you use the system for the first time or need an overview of its history, function and applications, you may visit our “Introduction” page first. If you have any questions, please get in touch.

In agreement with the FMP directors, we will charge will charge 1,000 Euro € for a CRISPR/Cas cell clone (knock-in or knock out) when the clone is confirmed.

In addition to CRISPR/Cas gene editing, the Cell Engineering Facility is also able to make stable transfected cell clones using various cell lines upon request. 

Introduction into CRISPR/Cas

Publications (via ORCID)

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The Pseudo signal peptide of the corticotropin-releasing factor receptor type 2A prevents receptor oligomerization

  • Teichmann, Anke; Rutz, Claudia; Kreuchwig, Annika; Krause, Gerd; Wiesner, Burkhard; Schülein, Ralf

The Journal of biological chemistry 2012

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The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression

  • Zampatis, Dimitris E; Rutz, Claudia; Furkert, Jens; Schmidt, Antje; Wüstenhagen, Doreen; Kubick, Stefan; Tsopanoglou, Nikos E; Schülein, Ralf

FEBS letters 2012

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Live cell imaging of G protein-coupled receptors

  • Teichmann, Anke; Schmidt, Antje; Wiesner, Burkhard; Oksche, Alexander; Schülein, Ralf

Methods in molecular biology (Clifton, N.J.) 2012

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Use of Kikume green-red fusions to study the influence of pharmacological chaperones on trafficking of G protein-coupled receptors

  • Ridelis, Ingrid; Schmidt, Antje; Teichmann, Anke; Furkert, Jens; Wiesner, Burkhard; Schülein, Ralf

FEBS letters 2012

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Functional significance of cleavable signal peptides of G protein-coupled receptors

  • Schülein, Ralf; Westendorf, Carolin; Krause, Gerd; Rosenthal, Walter

European journal of cell biology 2012

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The proteoglycan syndecan 4 regulates transient receptor potential canonical 6 channels via RhoA/Rho-associated protein kinase signaling

  • Liu, Ying; Echtermeyer, Frank; Thilo, Florian; Theilmeier, Gregor; Schmidt, Antje; Schülein, Ralf; Jensen, Boye L; Loddenkemper, Christoph; Jankowski, Vera; Marcussen, Niels; Gollasch, Maik; Arendshorst, William J; Tepel, Martin

Arteriosclerosis, thrombosis, and vascular biology 2012

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