Recent Publications

2024

Betül Haykir, Seraina Olivia Moser, Eva Maria Pastor-Arroyo, Udo Schnitzbauer, Zsuzsa Radvanyi, Isabel Prucker, Danye Qiu, Dorothea Fiedler, Adolfo Saiardi, Henning J. Jessen, Nati Hernando, Carsten A Wagner. The Ip6k1 and Ip6k2 Kinases Are Critical for Normal Renal Tubular Function. J. Am. Soc. Nephrol. 2024, (00):10.1681/ASN.0000000000000303.

Lin Fu, Jimin Du, David Furkert, Megan L. Shipton, Xiaoqi Liu, Tim Aguirre, Alfred C. Chin, Andrew M. Riley, Barry V. L. Potter, Dorothea Fiedler, Xu Zhang, Yi Zhu, Chenglai Fu. Depleting inositol pyrophosphate 5-InsP7 protected the heart against ischemia-reperfusion injury by elevating plasma adiponectinCardiovascular Research 2024, cvae017.

2023

Tim Aguirre, Gillian L. Dornan, Sarah Hostachy, Martin Neuenschwander, Carola Seyffarth, Volker Haucke, Anja Schütz, Jens Peter von Kries, Dorothea Fiedler. An unconventional gatekeeper mutation sensitizes inositol hexakisphosphate kinases to an allosteric inhibitor. eLife 2023, 12, RP88982.

Sarah Hostachy, Huanchen Wang, Guangning Zong, Katy Franke, Andrew M. Riley, Peter Schmieder, Barry V. L. Potter, Stephen B. Shears, Dorothea Fiedler. Fluorination Influences the Bioisostery of Myo-Inositol Pyrophosphate Analogs. Chem. Eur. J. 2023, e202302426.

Tim Aguirre, Ellen Teichmann, Florian Q. Römpp, Ruthey Vivier, Cole Bryant, Matthew A. Hulverson, Wesley C. Van Voorhis, Kayode K. Ojo, J. Stone Doggett, Dorothea Fiedler & Stefan Hecht. Photoswitchable Inhibitors to Optically Control Specific Kinase Activity. ACS Chem. Biol. 2023, 18 (6), 1378.

Joka Pipercevic, Bastian Kohl, Ruta Gerasimaite, Véronique Comte-Miserez, Sarah Hostachy, Thomas Müntener, Elia Agustoni, Henning J. Jessen, Dorothea Fiedler, Andreas Mayer & Sebastian Hiller. Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction. Nat. Commun. 2023, 14, 2645.

Ji Qi, Weiwei Cheng, Zhe Gao, Yuanyuan Chen, Megan L. Shipton, David Furkert, Alfred C. Chin, Andrew M. Riley, Dorothea Fiedler, Barry V.L. Potter, Chenglai Fu. Itraconazole inhibits endothelial cell migration by disrupting inositol pyrophosphate-dependent focal adhesion dynamics and cytoskeletal remodelingBiomed. Pharmacother. 2023, 161, 114449.

Leonie Kurz, Peter Schmieder, Nicolás Veiga & Dorothea Fiedler. One Scaffold, Two Conformations: The Ring-Flip of the Messenger InsP8 Occurs under Cytosolic Conditions. Biomolecules 202313 (4), 645.

Jacob Gorenflos, Peter Schmieder, Kristin Kemnitz-Hassanin, Hatice Ceyda Asikoglu, Arif Celik, Christian E. Stieger, Dorothea Fiedler, Stephan Hinderlich & Christian Hackenberger. Real-Time Monitoring of the Sialic acid Biosynthesis Pathway by NMR. Chem. Sci. 2023, 14, 3482–3492.

Nathaniel W. Brown, Sandra K. Schlomach, Alan M. Marmelstein, Dorothea Fiedler. Chemoselective Labeling and Immobilization of Phosphopeptides with Phosphorimidazolide Reagents. ChemBioChem 2023, 24, e2022004.

Soumyadip Sahu, Jacob Gordon, Chunfang Gu, Mack Sobhany, Dorothea Fiedler, Robin E. Stanley & Stephen B. Shears. Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP7Biomolecules 202313 (1), 153.

2022

Minh Nguyen Trung, Stefanie Kieninger, Zeinab Fandi, Danye Qiu, Guizhen Liu, Neelay K. Mehendale, Adolfo Saiardi, Henning Jessen, Bettina Keller, Dorothea Fiedler. Stable Isotopomers of myo-Inositol Uncover a Complex MINPP1-Dependent Inositol Phosphate Network. ACS Cent. Sci. 2022, 8, 12, 1683–1694.

Minh Nguyen Trung, David Furkert, Dorothea Fiedler. Versatile signaling mechanisms of inositol pyrophosphates. Curr. Opin. Chem. Biol. 2022, 70, 102177.

Natascha Andrea Kuenzel, Abel R. Alcázar-Román, Adolfo Saiardi, Simon M. Bartsch, Sarune Daunaraviciute, Dorothea Fiedler & Ursula Fleig. Inositol Pyrophosphate-Controlled Kinetochore Architecture and Mitotic Entry in S. pombe. J. Fungi 2022, 8 (9), 933.

Philipp Gaugler, Robin Schneider, Guizhen Liu, Danye Qiu, Jonathan Weber, Jochen Schmid, Nikolaus Jork, Markus Häner, Kevin Ritter, Nicolás Fernández-Rebollo, Ricardo F. H. Giehl, Minh Nguyen Trung, Ranjana Yadav, Dorothea Fiedler, Verena Gaugler, Henning J. Jessen, Gabriel Schaaf & Debabrata Laha. Arabidopsis PFA-DSP-Type Phosphohydrolases Target Specific Inositol Pyrophosphate Messengers. Biochemistry 2022, 61, 12, 1213–1227.

Christopher L. Sander, Jennings Luu, Kyumhyuk Kim, David Furkert, Kiyoung Jang, Joerg Reichenwallner, MinSoung Kang, Ho-Jun Lee, Bryan T. Eger, Hui-Woog Choe, Dorothea Fiedler, Oliver P. Ernst, Yong Ju Kim, Krzysztof Palczewski, Philip D. Kiser. Structural evidence for visual arrestin priming via complexation of phosphoinositols. Structure 2022, 30, 2, 263–277.

Tim Kröber, Simon Maria Bartsch, Dorothea Fiedler. Pharmacological tools to investigate inositol polyphosphate kinases – Enzymes of increasing therapeutic relevance. Adv. Biol. Regul. 2022, 83, 100836.

Yann Desfougères, Paloma Portela-Torres, Danye Qiu, Thomas M. Livermore, Robert K. Harmel, Filipy Borghi, Henning J. Jessen, Dorothea Fiedler, Adolfo Saiardi. The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis. Adv. Bio. Regul. 2022, 83, 100835.

2021

Esther Riemer, Danye Qiu, Debabrata Laha, Robert K. Harmel, Philipp Gaugler, Verena Gaugler, Michael Frei, Mohammad-Reza Hajirezaei, Nargis Parvin Laha,Lukas Krusenbaum, Robin Schneider, Adolfo Saiardi, Dorothea Fiedler, Henning J. Jessen, Gabriel Schaaf, Ricardo F.H. Giehl. ITPK1 is an InsP6/ADP phosphotransferase that controls phosphate signaling in Arabidopsis. Molecular Plant 2021, 14 (11), 1864–1880.

Daniel Couto, Annika Richter, Henriette Walter, David Furkert, Michael Hothorn & Dorothea Fiedler. Using Biotinylated Myo -Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate − Protein Interactions with Surface-Based Biosensors. ACS Biochemistry 2021, 60, 2739–2748.

Katrin Röhm, Fernando Gonzalez Uarquin, Robert K. Harmel, Minh Nguyen Trung, Martin Diener, Dorothea Fiedler, Korinna Huber, Jana Seifert. Investigation of a potential electrogenic transport-system for myo-inositol in the small intestine of laying hens. British Poultry Science 2021, 63 (1), 91–97.

Thi Phuong Nam Bui, Louise Mannerås-Holm, Robert Puschmann, Hao Wu, Antonio Dario Troise, Bart Nijsse, Sjef Boeren, Fredrik Bäckhed, Dorothea Fiedler, Willem M. deVos. Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health. Nat. Commun. 2021, 12, 4798.

Sarah Hostachy, Tillmann Utesch, Katy Franke, Gillian Leigh Dornan, David Furkert, Berke Türkaydin, Volker Haucke, Han Sun & Dorothea Fiedler. Dissecting the Activation of Insulin Degrading Enzyme by Inositol Pyrophosphates and Their Bisphosphonate Analogs. Chem. Sci. 2021, 12, 10696–10702.

Brian S. Mantilla, Karunakaran Kalesh, Nathaniel W. Brown Jr, Dorothea Fiedler, Roberto Docampo. Affinity‐based proteomics reveals novel targets of inositol pyrophosphate (5‐IP7)‐dependent phosphorylation and binding in Trypanosoma cruzi replicative stages. Mol. Microbiol. 2021, 115, 986–1004.

David Furkert, Michal Nadler-Holly, Dorothea Fiedler. Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes. STAR Protocols 2021, 2, 100277.

Gangling Liao, Wenjuan Ye, Tyler Heitmann, Glen Ernst, Michael DePasquale, Laiyi Xu, Michael Wormald, Xin Hu, Marc Ferrer, Robert K. Harmel, Dorothea Fiedler, James Barrow & Huijun Wei. Identification of Small-Molecule Inhibitors of Human Inositol Hexakisphosphate Kinases by High-Throughput Screening. ACS Pharmacol. Transl. Sci. 2021, 4, 2, 780–789.

Guangning Zong, Nikolaus Jork, Sarah Hostachy, Dorothea Fiedler, Henning J. Jessen, Stephen B. Shears & Huanchen Wang. New Structural Insights Reveal an Expanded Reaction Cycle for Inositol Pyrophosphate Hydrolysis by Human DIPP1. The FASEB Journal 2021, 2 (35), 1–14.

Martina K. Ried, Rebekka Wild, Jinsheng Zhu, Joka Pipercevic, Kristina Sturm, Larissa Broger, Robert K Harmel, Luciano A Abriata, Ludwig A Hothorn, Dorothea Fiedler, Sebastian Hiller, Michael Hothorn. Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis. Nat. Commun. 2021, 12, 384.

2020

Danye Qiu, Miranda S. Wilson, Verena B. Eisenbeis, Robert K. Harmel, Esther Riemer, Thomas M. Haas, Christopher Wittwer, Nikolaus Jork, Chunfang Gu, Stephen B. Shears, Gabriel Schaaf, Bernd Kammerer, Dorothea Fiedler, Adolfo Saiardi & Henning J. Jessen. Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry. Nat. Commun. 2020, 11, 6035.

Alfred C. Chin, Zhe Gao, Andrew M. Riley, David Furkert, Christopher Wittwer, Amit Dutta, Tomas Rojas, Evan R. Semenza, Robin A. Felder, Jennifer L. Pluznick, Henning J. Jessen, Dorothea Fiedler, Barry V. L. Potter, Solomon H. Snyder, Chenglai Fu. The inositol pyrophosphate 5-InsP7 drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α. Science Advances 2020, 6 (44).

Desmarini Desmarini, Sophie Lev, David Furkert, Ben Crossett, Adolfo Saiardi, Keren Kaufman-Francis, Cecilia Li, Tania C. Sorrell, Lorna Wilkinson-White, Jacqueline Matthews, Dorothea Fiedler, Julianne Teresa Djordjevic. IP7-SPX domain interaction controls fungal virulence by stabilizing phosphate signaling machinery. mBio 2020, 11 (5).

Feng Rao, Jiyoung Cha, Jing Xu, Risheng Xu, M. Scott Vandiver, Richa Tyagi, Robert Tokhunts, Michael A. Koldobskiy, Chenglai Fu, Roxanne Barrow, Mingxuan Wu, Dorothea Fiedler, James C. Barrow, Solomon H. Snyder. Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2. Mol. Cell 2020, 79 (4), 702.

David Furkert, Sarah Hostachy, Michal Nadler-Holly, Dorothea Fiedler. Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome. Cell Chem. Bio. 2020, 27 (8), 1097–1108.

Soumyadip Sahu, Zhenzhen Wang, Xinfu Jiao, Chunfang Gu, Nikolaus Jork, Christopher Wittwer, Xingyao Li, Sarah Hostachy, Dorothea Fiedler, Huanchen Wang, Henning J. Jessen, Megerditch Kiledjian, Stephen B. Shears. InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics. PNAS 2020, 117 (32), 19245–19253.

Mao Li, Robert Puschmann, Andreas Herdlitschka, Dorothea Fiedler, Helma Wennemers. Delivery of myo‐Inositol Hexakisphosphate to the Cell Nucleus with a Proline‐Based Cell‐Penetrating Peptide. Angew. Chem. Int. Ed. 2020, 59, 15586–15589.

Robert Puschmann, Robert K. Harmel, Dorothea Fiedler. Analysis of metabolically labeled inositol phosphate messengers by NMR. Methods Enzymol. 2020, 641, 35–52.

Xingyao Li, Chunfang Gu, Sarah Hostachy, Soumyadip Sahu, Christopher Wittwer, Henning J. Jessen, Dorothea Fiedler, Huanchen Wang, Stephen B. Shears. Control of XPR1-dependent cellular phosphate efflux by InsP8 is an exemplar for functionally-exclusive inositol pyrophosphate signaling. PNAS 2020, 117 (7), 3568–3574.

2019

Robert Puschmann, Robert K. Harmel & Dorothea Fiedler. Scalable Chemoenzymatic Synthesis of Inositol Pyrophosphates. Biochemistry 2019, 58, 38, 3927–3932.

Jinsheng Zhu, Kelvin Lau, Robert Puschmann, Robert K. Harmel, Youjun Zhang, Verena Pries, Philipp Gaugler, Larissa Broger, Amit K. Dutta, Henning J. Jessen, Gabriel Schaaf, Alisdair R. Fernie, Ludwig A. Hothorn, Dorothea Fiedler, Michael Hothorn. Two bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis. eLife 2019, 8:e43582

Martin Penkert, Anett Hauser, Robert Harmel, Dorothea Fiedler, Christian P. R. Hackenberger, Eberhard Krause. Electron Transfer/Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations. J. Am. Soc. Mass Spectrom. 2019, 30, 1578–1585.

Robert K. Harmel, Robert Puschmann, Minh Nguyen Trung, Adolfo Saiardi, Peter Schmieder, Dorothea Fiedler. Harnessing 13C-labeled myo-inositol to interrogate inositol phosphate messengers by NMR. Chem. Sci. 2019, 10, 5267–5274.

2013 - 2018

2018

Alan M. Marmelstein, Jeremy A.M. Morgan, Martin Penkert, Daniel T. Rogerson, Eberhard Krause, Jason W. Chin & Dorothea Fiedler. Pyrophosphorylation via Selective Phosphoprotein Derivatization. Chem. Sci. 2018, 9, 5929–3596.

Cole M. Dovey, Jonathan Diep, Bradley P. Clarke, Andrew T. Hale, Dan E. McNamara, Hongyan Guo, Nathaniel W. Brown Jr., Jennifer Yinuo Cao, Christy R. Grace, Peter J. Gough, John Bertin, Scott J. Dixon, Dorothea Fiedler, Edward S. Mocarski, William J. Kaiser, Tudor Moldoveanu, John D. York, Jan E. Carette. MLKL Requires the Inositol Phosphate Code to Execute Necroptosis. Mol. Cell 2018, 70 (5), 936–948.

Robert Harmel & Dorothea Fiedler. Features and regulation of non-enzymatic post-translational modifications. Nature Chem. Bio. 2018, 14, 244–252.

2017

Martin Penkert, Lisa M. Yates, Michael Schümann, David Perlman, Dorothea Fiedler, Eberhard Krause. Unambiguous Identification of Serine and Threonine Pyrophosphorylation Using Neutral-Loss-Triggered Electron-Transfer/Higher-Energy Collision Dissociation. Anal. Chem. 2017, 89 (6), 3672–3680.

Alan M. Marmelstein, Javier Moreno, Dorothea Fiedler. Chemical Approaches to Studying Labile Amino Acid Phosphorylation. Top. Curr. Chem. 2017, 375:22.

2016

Mingxuan Wu, Lucy S. Chong, David H. Perlman, Adam C. Resnick, Dorothea Fiedler. Inositol Polyphosphates intersect with signalling and metabolic networks via two distinct mechanisms. PNAS 2016, 113 (44), E6757–6765.

Manasa Chanduri, Ashim Rai, Aushaq Bashir Malla, Mingxuan Wu, Dorothea Fiedler, Roop Mallik, Rashna Bhandari. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem. J. 2016, 473 (19), 3031–3047.

Anastasia Hager, Mingxuan Wu, Huanchen Wang, Nathaniel W. Brown Jr., Stephen B Shears, Nicolás Veiga, Dorothea Fiedler. Cellular Cations Control Conformational Switching of Inositol Pyrophosphate Analogues. Chem. Eur. J. 2016, 22 (35), 12406–12414.

Nathaniel W. Brown Jr., Alan M. Marmelstein, Dorothea Fiedler. Chemical tools for interrogating inositol pyrophosphate structure and function. Chem. Soc. Rev. 2016, 45, 6311–6326.

Elizabeth A. Steidle, Lucy S. Chong, Mingxuan Wu, Elliot Crooke, Dorothea Fielder, Adam C. Resnick, Ronda J. Rolfes. A novel inostiol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 2-diphosphoinositol pentakisphosphate (5PP-IP5). J. Biol. Chem. 2016, 291 (13), 6772–6783.

Lisa M. Yates & Dorothea Fiedler. A Stable Pyrophosphoserin Analog for Incorporation into Peptides and Proteins. ACS Chem. Biol. 2016, 11 (4), 1066–1073.

2015

Florence J. Williams & Dorothea Fiedler. A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins. ACS Chem. Biol. 2015, 10 (9), 1958–1963.

Humberto Martin, Michael Shales, Pablo Fernandez-Piñar, Ping Wei, Maria Molina, Dorothea Fiedler, Kevan M. Shokat, Pedro Beltrao, Wendell Lim, Nevan J. Krogan. Differential genetic interactions of yeast stress response MAPK pathways. Mol. Syst. Biol. 2015, 11:800.

John H. Conway & Dorothea Fiedler. An Affinity Reagent for the Recognition of Pyrophosphorylated Peptides. Angew. Chem. Int. Ed. 2015, 54, 3941–3945.

Lisa M. Yates & Dorothea Fiedler. Establishing the Stability and Reversability of Protein Pyrophosphorylation with Synthetic Peptides. ChemBioChem 2015, 16, 415–423.

2014

Lisa M. Yates, Alan M. Marmelstein & Dorothea Fiedler. Synthetic Pyrophosphorylation Methods and Their Use in Chemical Biology. Synlett 2014, 25 (16), 2239–2245.

Mingxuan Wu, Lucy S. Chong, Samanta Capolicchio, Henning J. Jessen, Adam C. Resnick & Dorothea Fiedler. Elucidating Diphosphoinositol Polyphosphate Function with Nonhydrolyzable Analogues. Angew. Chem. Int. Ed. 2014, 53, 7192–7197.

Feng Roa, Jiyoung Cha, Jing Xu, Risheng Xu, M. Scott Vandiver, Richa Tyagi, Robert Tokhunts, Michael A. Koldobskiy, Chenglai Fu, Roxanne Barrow, Mingxuan Wu, Dorothea Fiedler, James C. Barrow, Solomon H. Snyder. Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tti2. Mol. Cell 2014, 54, 119–132.

Alan M. Marmelstein, Lisa M. Yates, John H. Conway & Dorothea Fiedler. Chemical Pyrophosphorylation of Functionally Diverse Peptides. J. Am. Chem. Soc. 2014, 136, 108–111.

2013

Joseph I. Kliegman, Dorothea Fiedler, Colm J. Ryan, Yi-Fan Xu, Xiao-yang Su, David Thomas, Max C. Caccese, Ada Cheng, Michael Shales, Joshua D. Rabinowitz, Nevan J. Krogan, Kevan M. Shokat. Chemical Genetics of Rapamycin-Insensitive TORC2 in S. cerevisiae. Cell Rep. 2013, 5 (6), 1725–1736.

Mingxuan Wu, Barbara E. Dul, Alexandra J. Trevisan & Dorothea Fiedler. Synthesis and characterization of non-hydrolysable diphosphoinositol polyphosphate messengers. Chem. Sci. 2013, 4, 405–410.