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The core facility aims at providing simple synthetic peptides to FMP research groups.
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The core facility was formed in February 2013 as a service unit for peptide synthesis and is part of the department of „Biomolecule Modification and Delivery“. Christian Hackenberger contributes his expertise for the synthesis of advanced post-translationally modified polypeptides as well as unnatural peptide –conjugates and Ines Kretzschmar takes care for the execution of the peptide synthesis procedure.
Our aim is to provide synthetic peptides to all research groups at the FMP. The facility is equipped with two parallel peptide synthesizers and a microwave-assisted synthesizer for challenging peptide sequences, one analytical and one preparative RP-HPLC instruments as well as a MALDI-ToF mass spectrometer.
The full repertoire of standard SPPS methods is employed in this facility to generate linear peptides with or without side chain and / or termini-modifications. More challenging peptides are also made including dye-labeled peptides, head-to-tail or side chain-to-side chain cyclic peptides with and without dye-labeling. Furthermore, peptides containing unnatural amino acids, building blocks or proline-mimetics, D-amino acids, polyethylene glycol chains and phosphorylated amino acids are synthesized. Up to now this facility synthesized 2433 peptides (until 2023), an average of approx. 250 peptides/year.
In addition to its services, this core facility is embedded in several FMP research projects and we process orders from Fiedler lab, Broichhagen lab, Lehmann/Haucke lab and Lange lab. Additionally, we optimize the synthesis of cyclic cell-penetrating peptides (cCPPs), which have been applied to the delivery of functional nanobodies into specific intracellular compartments by the Hackenberger lab.
Please fill in the peptide synthesis order form to submit your sequence and send the form by email to Ines Kretzschmar.
People
Prof. Dr. Christian Hackenberger studied chemistry at the University of Freiburg, at the RWTH Aachen and at the University of Wisconsin/Madison (USA). Since 2012, he is Leibniz-Humboldt Professor of Chemical Biology at Humboldt University and Head of the Chemical Biology II Department at the FMP. He is the founder of Tubulis GmbH, a company that develops new, better-tolerated therapeutics against cancer.
Ines was born in the north of Brandenburg, grew up in a large district town in the Free State of Saxony, spent the storm and stress period (studies) in Berlin and stayed there.
She has been trying to produce peptides since 1998, first at the Charité and since 2013 in the Core Facility at the FMP. It doesn't always succeed, but that's rarely her fault (never, actually).
To escape the lab routine, she likes to ride her motorcycle (for the fresh air) and dedicate herself to her allotment (against the lab pallor).
If there is still time, she likes to go bowling.
The Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) is part of the Forschungsverbund Berlin e.V. (FVB), which legally represents seven non-university research institutes - members of the Leibniz Association - in Berlin.
Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10,
13125 Berlin, Germany
