Development of new unnaturally modified glycoproteins
In collaboration with Prof. Stephan Hinderlich (Beuth-Hochschule) we recently expanded repertoire of unnaturally modified glycoproteins by metabolic oligosaccharide engineering. For this purpose C4-substituted ManNAc-derivatives were synthesized and incorporated into novel C7-modified sialic acids in glycoproteins in cells and in living zebrafish (Scheme 2). These novel biopolymers will be investigated for the development of new diagnostic and therapeutic glycoproteins and potentially applied to the in vivo modification of living animal models.
(Reference: H. Möller, V. Böhrsch, J. Bentrop, J. Bender, S. Hinderlich,* C. P. R. Hackenberger*
Angew. Chem. 2012, 124, 6088 –6092
Angew. Chem. Int. Ed. 2012, 51, 5986 –599
Glycan-Specific Metabolic Oligosaccharide Engineering of C7-Substituted Sialic Acids)