Research groupResearch Unit

Christian Hackenberger

Biomolecule Modification and Delivery

Portrait

Our group is interested in understanding how nature generates specific biological function in complex cellular environments. Research projects in our laboratory combine techniques and approaches from organic chemistry, biochemistry and biophysics with the major emphasis on synthetic methodology development for natural protein modifications.

Welcome!

We are a young interdisciplinary research group at FMP. Our research projects focus on understanding how nature generates specific biological function in complex cellular environments. Research projects in our laboratory combine techniques and approaches from organic chemistry, biochemistry and biophysics with the major emphasis on synthetic methodology development for natural protein modifications. For more information please visit the corresponding sections in this homepage.

How proteins can change fate

In the cell, protein modifications control many signaling pathways that support healthy functioning and that are disrupted in disease. Such modifications can act as “toggle switches” between health and disease. Chemical biologists want to control these protein modifications in the cell, both to study the biological role of such modifications and to decorate proteins with fluorescent moieties that permit their visualization. Our laboratory aims to identify new chemical approaches that allow the modification of peptides and proteins, both on isolated biomolecules as well as in living cells and organisms. Here, our main objective is to apply these highly selective bioconjugation reactions to study the functional consequences of natural protein modifications, as well as to generate novel pharmaceutical and medicinal applications, such as protein or antibody-drug conjugates (ADCs) against cancer or viral infections.

 

Contact Offce

Jennifer Trümpler

Secretary, Hackenberger Group,
Secretary Fiedler Group

Contact Office and Graduate School

Katrin Wittig

Administrative Assistent, Hackenberger Group,
PhD Program Coordinator


Research Section

Chemical Biology

Patents

Bifunctional peptide with mucoadhesive and virus binding properties
WO002023187142A1

Novel FLT3 antibodies and antibody-drug-conjugates based thereon, therapeutig methods and uses thereof in combination with tyrosine kinase inhibitors
WO002023105087A1

Conjugates comprising a phosphorus (V) and a drug moiety
WO002023083900A1

Thiol-conjugation with unsaturated phosphorus(V) compounds
WO002022223783A1

Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additivesS
WO002022180242A2

Improved CD30 targeting antibody drug conjugates and uses thereof
WO002022136555A1

Chemoselective Thiol-Conjugation with Alkene or Alkyne-Phosphonothiolates and -Phosphonates
WO002019170710A2

Chemoselective Thiol-Conjugation with Alkene or Alkyne-Phosponamidates
WO002018041985A1

Unusual substrates of tubulin tyrosine ligase
WO002017186855A1

Means and Methods for the site-specific functionalization of polypeptides
WO002016066749A1

Compound modified by a phosphoramidate and/or phosphonamide group and use thereof
WO2010102997A3

Group Members

 People

By PositionA-Z
  • Prof. Dr. Christian Hackenberger studied chemistry at the University of Freiburg, at the RWTH Aachen and at the University of Wisconsin/Madison (USA). Since 2012, he is Leibniz-Humboldt Professor of Chemical Biology at Humboldt University and Head of the Chemical Biology II Department at the FMP. He is the founder of Tubulis GmbH, a company that develops new, better-tolerated therapeutics against cancer.

  • Katta was born in West Berlin into an original Berlin family.  After a professional training in a large law firm, she thought that studying law would be a good idea. But it wasn't...
    Even a change to archaeology wasn't much better, so she gave up studying and took other paths.
    She has been working for Christian since 2008 and moved with him from FU Berlin to FMP in 2013.
    Katta only has a part-time job because otherwise she wouldn't have enough time for all her other interests. In addition to her hobbies of sewing, scouting and softball, she is involved in raising awareness for people with Down syndrome because her daughter is affected by it.

  • Jenny grew up in the most “chemical” part of Germany near the Leuna- and Buna-plants. However, she decided to become an animal farmer first. After working in an Irish company for some years, she went back to school to be a bilingual secretary. Assisting the management of a consulting company in Berlin since 2010, she made time to study three evenings a week and graduated in business administration (B.A.). Having found her interest in academia, she switched to the International Office at Eberswalde University for Sustainable Development to support students going abroad and international students studying here. She is happy to assist the Fiedler and Hackenberger group in administrative matters. Chocolate is vital for her, she always stores a pile or two in her desk.

  • Growing up in the beautiful state of Brandenburg, Kristin trained as a medical laboratory assistant in Schwerin after graduating from high school. Afterwards, she worked for several years in a medical-diagnostic laboratory in the middle of Berlin, a metropolis that was very lively for her.
    The desire to develop herself, to have new challenges, to acquire knowledge and new techniques led her into research and finally to the FMP.
    Finally out of the big city and back to her quiet home. Because if you don't have to go to the city for work, you can also move back to the Brandenburg countryside.
    Since the end of 2014, she has been taking care of the biochemical laboratory in Christian Hackenberger's group as a technical assistant: she provides practical advice or assistance to anyone who wants it. She "feeds" the cell culture, our cabinets and shelves and many times a data- and protein-hungry colleague. She pipettes, mutates, expresses, optimizes and explodes... Upsi! ;-) The latter only in exceptional cases... and precisely because not everything always goes according to plan in the research lab. But otherwise it would be boring!

  • Beate was born and raised in Berlin, spent half a year in Australia and then worked for almost two decades in green and white biotechnology. Since 2018 at the FMP, she is acting as "HPLC specialist" and contact person for many other technical-organizational worries and needs in the working group.

  • Ines was born in the north of Brandenburg, grew up in a large district town in the Free State of Saxony, spent the storm and stress period (studies) in Berlin and stayed there.
    She has been trying to produce peptides since 1998, first at the Charité and since 2013 in the Core Facility at the FMP. It doesn't always succeed, but that's rarely her fault (never, actually).
    To escape the lab routine, she likes to ride her motorcycle (for the fresh air) and dedicate herself to her allotment (against the lab pallor).
    If there is still time, she likes to go bowling.

  • Bingjia Yan obtained his B.S. degree from the Beijing Institute of Petrochemical Technology (China) in 2011. After obtaining his PhD from the University of Southampton (UK) in 2016 under the supervision of Prof. Martin Grossel and Prof. Simon Coles, he returned to China and conducted postdoctoral research in the laboratory of Prof. Lei Liu in the Department of Chemistry at Tsinghua University (China). Then, he moved to Berlin and joined Prof. Christian Hackenberger's group at FMP until now. He is interested in chemical protein synthesis and its applications to biomedical studies.

  • Originally from sunny Philippines, Vince obtained his PhD from Kyoto University, Japan under Prof. Shiroh Futaki funded by the MEXT scholarship. He worked on a peptide-based strategy that combines macropinocytosis induction and endosome release for intracellular delivery of biomacromolecules. Wanting to learn more chemistry, Vince moved to the other side of the world to be a postdoc in the Hackenberger group. He performs microscopy techniques to study the cellular entry mechanisms and to fine-tune the efficacies of cell-penetrating peptides (CPP) and CPP-additives. Away from the lab, he enjoys cooking Asian dishes, drinks, and pub quizzes.

  • Mark studied Bio-Pharmaceutical Sciences (BSc) and Chemistry (MSc) at Leiden University, where he worked on total synthesis (Prof. Dr. Gijs van der Marel, Prof. Dr. Jeroen Codée) and activity-based protein profiling (prof. Dr. Hans Aerts) during internships. During his PhD with Dr. Sander van Kasteren and Prof. Dr. Herman Overkleeft at Leiden University, he worked on trans-cyclooctene/tetrazine ‘click to release’ bioorthogonal chemistry, specifically concerning the development of new synthetic strategies and applications of this method towards chemical immunology. Mark moved to Berlin in 2021, where he is developing site-specific protein modification chemistry and exploring new fundamental concepts of antibody-drug conjugates (ADCs) as a postdoctoral fellow in the group of Prof. Dr. Christian Hackenberger at the Leibniz Institute for Molecular Pharmacology (FMP).

  • Leander holds an MChem in Chemistry from University of Southampton and during his studies did Master’s Research Placement at University of Toronto in Andrew Wooley’s group working on photoswitchable affinity proteins. He then moved to the University of Cambridge for PhD research in the development of nanostructured photocatalysts derived from riboflavin (vitamin B2).

  • Alastair obtained his MChem from the University of Manchester where he remained to continue his PhD. He worked within in the group of Daniele Leonori on various projects centred around the use of radical and photochemistry in new methodology development. During an extension year, after the group’s move to RWTH Aachen, he worked on a side project involving unnatural amino acids which inspired him to pivot towards chemical biology research for his postdoc. Outside the lab he enjoys finding new venues to see concerts at, bouldering and listening to music.  

  • Originally from Dublin, Ireland - Luke studied Biotechnology (B.Sc.) at Maynooth University, where he stayed to carry out a PhD in Supramolecular Organic Chemistry under the supervision of Dr Robert Elmes. Luke’s work mainly concerned the synthesis and evaluation of anionophores, and fluorescent/luminescent probes for the treatment and diagnosis of antimicrobial-resistant infections. Luke hopes to further explore anti-infectives and chemical probes during his postdoc at the FMP, through the lens of Phosphorous and peptide chemistry. When he isn’t in the lab, Luke can likely be found out for a run around Berlin, or in his local bouldering/Brazilian jiu-jitsu gym! Aside from sport, Luke enjoys exploring the city through its many amazing restaurants, cafés and flea markets.

  • Sebastián comes from one of the most diverse countries in the world, Colombia, and for this reason he loves nature and the ocean. Therefore, his scientific interests are linked to natural products synthesis, biochemistry, chemical biology and biocatalysis. He has traveled far and wide through Latinamerica and enjoy very much typical mexican food. His researching goals include becoming a professor in a public university in his country, as he deeply believes that through education true lasting changes can be made in society. In his free time he enjoys biking through new streets in Berlin, listening, playing and dancing to cool music.

  • Eleftheria grew up in Greece, where she completed her B.Sc. in Chemistry. After that, she decided she really had enough with all the sun and sea, so she moved to Germany, where she did her master’s in molecular science at the FAU in Erlangen. During this time Elef gained a lot of interest in chemical biology (apart from Bavarian beer!) so she joined the Hackenbees for her master thesis. Little did she know, she would stay there for a few years, to develop chemical probes for studying functional protein phosphatases. In her free time, Elef likes to meet with friends, dance (even if she is alone in her room) and researching about how she could make her own fashion clothing line!

  • Luise was born in beautiful Hamburg. She studied Biochemistry at the Freie Universität Berlin. After exploring the field of toxicology during her bachelor thesis, where she studied the effects of  pyrrolizidine alkaloids on liver cells and a brief stay in tissue engineering where she worked on producing artificial blood vessels she visited the Hackenberger Group for a research internship. There she learned about chemical biology and especially cell penetrating peptides for the first time and continued working on this topic for her master thesis. She stayed with the Hackenberger lab for the opportunity to further optimize CPPs and CPP mediated delivery of protein cargo.

  • Marc grew up in the suburbs of Munich, Bavariva and completed his undergrads in pharmacy at the LMU in Großhadern. During his studies, he started to gain interest in organic synthesis at Dr. Oliver Thorn-Seshold’s lab synthesizing covalent phostoswitchable microtubule disruptors and the scale up for in-vivo­ mice studies. After finishing his state examination, he joined David Spring’s lab in Cambridge, UK. There, he started out synthesizing unnatural amino acids for the incorporation of stapled peptides. He currently is working on a system to induce cleavage of phosphonamidates based bioconjugates. In his free time, Marc likes to dive into the Berlin nightlife, check out new art galleries or update his wardrobe at flee markets.

  • Sarah grew up in Flensburg and studied chemistry at the Humboldt University in Berlin. She joined the Hackenberger Lab for a Research Internship in the Summer of 2020. After enjoying her stay, she decided to stick around for her master´s thesis and PhD. Currently, she focusses on the synthesis of electrophilic and cell-surface reactive peptides and their application as additives in the cellular delivery of proteins. In her free time, she enjoys biking and running.

  • Judith grew up in beautiful Mainz on the Rhine before she pursued her scientific career in East Germany: She completed her bachelor at TU Dresden working on peptide-drug conjugates in Carsten Werner’s group and moved to Berlin for her M.Sc. degree at Humboldt-University. During a research project in the Ralser Lab/MS Core Facility at Charité she explored prospects of MS based assays for clinical diagnoses. After graduating, she decided to stay with the Hackenberger Lab where she also did her master thesis. Here, she designs cell-permeable conjugates for light induced protein labeling and looks into endogenous cysteine phosphorylation.

  • After spending the first 18 years of my life in Dresden, I started studying Biotechnical Chemistry at the TU Ilmenau. In the Master "Chemical Biology" in Jena, I specialized in the production and use of chemical tools in biological systems. In my final thesis, I investigated the mechanism of a natural product biosynthesis step in Christian Hertweck's laboratory at HKI Jena. In May 2022, I moved to Berlin to do research as a PhD candidate in Prof. Hackenberger's lab. In my project, I am working on making peptide electrophiles intracellularly available using cell-penetrating peptides. Outside the lab, I love doing sports, enjoy cooking and discovering the diversity of Berlin.

  • Juliana comes from the U.S. and completed her bachelor’s in Biochemistry at the University of Delaware. Before thinking of a further academic career in Germany, she gained experience in industry after graduation as an associate scientist in the field of translational medicine. She completed her master’s degree in Biochemistry at the Freie Universität Berlin and worked on exciting research projects centered mainly on protein-based targeted therapies as part of the Seeburger group, and assay investigation and design for a rare disease-linked enzyme as part of the AK Arenz group at Humboldt-Universität zu Berlin. With scientific interests that lie mainly in the field of chemical biology and protein modification, as well as method development - the Hackenbees have accepted her as one of their own (and her pup Mando). In her free time she is an avid functional fitness enthusiast, enjoys playing games, likes American style craft beers, and grooving to many genres of music!

  • I come from Japan and I finished my B.Sc. Biochemistry at Jacobs University Bremen. I'm currently in a gap year trying to figure out what I would like to study for masters. Christian was generous to offer me a position where I can broaden the scope to chemical biology under Vince and Chris as their minion, and I love working in the lab and am considering pursuing masters in chemical biology. After Masters and working for some years, I would like to retire and become a farmer in a Georgian mountain.

Job offers

Interested in an exciting interdisciplinary research project at the interface of organic chemistry, biochemistry and biophysics? For applications to internships, master theses, postdoctoral or PhD positions please contact Prof. Hackenberger (hackenbe(at)fmp-berlin.de) directly. Applicants should have a strong background in synthetic organic chemistry and/or biochemistry. Please send your application including a CV, a letter of motivation, a summery of your previous research experience and at least two letters of recommendation.

Funding

Deutsche Forschungsgemeinschaft (DFG)
SFB 1449 - Dynamic Hydrogels at Biointerfaces
GRK 2473 - SynPepBio
SFB 1349 - Fluorine-Specific Interactions

ANR/DFG (Project franco-allemands)
Intrazelluläres Targeting von Tau-spezifischen Einzeldomänen-Antikörpern

German Federal Ministry of Science (BMBF)
16GW0357K Konjugation und ADC-Synthese

Leibniz Association
SAW T85/2020 - Selective depletion of plasma cells secreting pathogenic autoantibodies in chronic autoimmune diseases

SprinD
Challenge "Neue Antivirale Mittel"

European Union
HORIZON-MSCA-2022-DN-01 - Doctoral training network TAu imMunE: strategies for innovative diagnosis and therapies in dementia (TAME)


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