Sigrid Milles

Nuclear magnetic resonance/single molecule fluorescence combinations to study dynamic protein systems

• Ida Marie Vedel; Andromachi Papagiannoula; Samuel Naudi-Fabra; Sigrid Milles

Current Opinion in Structural Biology 2023

NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins

• Aldo R. Camacho-Zarco; Vincent Schnapka; Serafima Guseva; Anton Abyzov; Wiktor Adamski; Sigrid Milles; Malene Ringkjøbing Jensen; Lukas Zidek; Nicola Salvi; Martin Blackledge

Chemical Reviews 2022

Quantitative Description of Intrinsically Disordered Proteins Using Single-Molecule FRET, NMR, and SAXS

• Samuel Naudi-Fabra; Maud Tengo; Malene Ringkjøbing Jensen; Martin Blackledge; Sigrid Milles

Journal of the American Chemical Society 2021

Synergies of Single Molecule Fluorescence and NMR for the Study of Intrinsically Disordered Proteins.

• Naudi-Fabra S; Blackledge M; Milles S

Biomolecules 2021

A Unified Description of Intrinsically Disordered Protein Dynamics under Physiological Conditions Using NMR Spectroscopy.

• Adamski W; Salvi N; Maurin D; Magnat J; Milles S; Jensen MR; Abyzov A; Moreau CJ; Blackledge M

Journal of the American Chemical Society 2019

The Nucleoprotein and Phosphoprotein of Measles Virus.

• Guseva S; Milles S; Blackledge M; Ruigrok RWH

Frontiers in microbiology 2019

Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.

• Desfosses A; Milles S; Jensen MR; Guseva S; Colletier JP; Maurin D; Schoehn G; Gutsche I; Ruigrok RWH; Blackledge M

Proceedings of the National Academy of Sciences of the United States of America 2019

An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function

• Sigrid Milles; Malene Ringkjøbing Jensen; Carine Lazert; Serafima Guseva; Stefaniia Ivashchenko; Guillaume Communie; Damien Maurin; Denis Gerlier; Rob W. H. Ruigrok; Martin Blackledge

Science Advances 2018

Characterization of intrinsically disordered proteins and their dynamic complexes: From in vitro to cell-like environments.

• Milles S; Salvi N; Blackledge M; Jensen MR

Progress in nuclear magnetic resonance spectroscopy 2018

Deciphering the Dynamic Interaction Profile of an Intrinsically Disordered Protein by NMR Exchange Spectroscopy.

• Delaforge E; Kragelj J; Tengo L; Palencia A; Milles S; Bouvignies G; Salvi N; Blackledge M; Jensen MR

Journal of the American Chemical Society 2018

Structural analysis of the complex between influenza B nucleoprotein and human importin-α.

• Labaronne A; Milles S; Donchet A; Jensen MR; Blackledge M; Bourhis JM; Ruigrok RWH; Crépin T

Scientific reports 2017

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.

• Fuertes G; Banterle N; Ruff KM; Chowdhury A; Mercadante D; Koehler C; Kachala M; Estrada Girona G; Milles S; Mishra A; Onck PR; Gräter F; Lemke EA

Proceedings of the National Academy of Sciences of the United States of America 2017

Investigating the Role of Large-Scale Domain Dynamics in Protein-Protein Interactions.

• Delaforge E; Milles S; Huang JR; Bouvier D; Jensen MR; Sattler M; Hart DJ; Blackledge M

Frontiers in molecular biosciences 2016

Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence Dependence.

• Milles S; Jensen MR; Communie G; Maurin D; Schoehn G; Ruigrok RW; Blackledge M

Angewandte Chemie (International ed. in English) 2016

Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.

• Delaforge E; Milles S; Bouvignies G; Bouvier D; Boivin S; Salvi N; Maurin D; Martel A; Round A; Lemke EA; Jensen MR; Hart DJ; Blackledge M

Journal of the American Chemical Society 2015

Plasticity of an ultrafast interaction between nucleoporins and nuclear transport receptors.

• Milles S; Mercadante D; Aramburu IV; Jensen MR; Banterle N; Koehler C; Tyagi S; Clarke J; Shammas SL; Blackledge M; Gräter F; Lemke EA

Cell 2015

Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields.

• Mercadante D; Milles S; Fuertes G; Svergun DI; Lemke EA; Gräter F

The journal of physical chemistry. B 2015

Mapping multivalency and differential affinities within large intrinsically disordered protein complexes with segmental motion analysis.

• Milles S; Lemke EA

Angewandte Chemie (International ed. in English) 2014

Continuous throughput and long-term observation of single-molecule FRET without immobilization.

• Tyagi S; VanDelinder V; Banterle N; Fuertes G; Milles S; Agez M; Lemke EA

Nature methods 2014

Organization of fluorescent cholesterol analogs in lipid bilayers - lessons from cyclodextrin extraction.

• Milles S; Meyer T; Scheidt HA; Schwarzer R; Thomas L; Marek M; Szente L; Bittman R; Herrmann A; Günther Pomorski T; Huster D; Müller P

Biochimica et biophysica acta 2013

Genetically encoded copper-free click chemistry.

• Plass T; Milles S; Koehler C; Schultz C; Lemke EA

Angewandte Chemie (International ed. in English) 2011

Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153.

• Milles S; Lemke EA

Biophysical journal 2011

The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.

• Marek M; Milles S; Schreiber G; Daleke DL; Dittmar G; Herrmann A; Müller P; Pomorski TG

The Journal of biological chemistry 2011

Solubilization of protein aggregates by the acid stress chaperones HdeA and HdeB.

• Malki A; Le HT; Milles S; Kern R; Caldas T; Abdallah J; Richarme G

The Journal of biological chemistry 2008