Johannes Broichhagen

Penta‐ALFA‐Tagged Substrates for Self‐Labelling Tags Allow Signal Enhancement in Microscopy

• Souvik Ghosh; Ramona Birke; Ashwin Karthick Natarajan; Johannes Broichhagen

Journal of Peptide Science 2025

Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization

• Kilian Roßmann; Ramona Birke; Joshua Levitz; Ben Jones; Johannes Broichhagen

RSC Chemical Biology 2025

Chemical probes for imaging cellular compartmentalization

• Margret H. Bülow; Johannes Broichhagen

Trends in Biochemical Sciences 2025

Deuterated oxazines are bright near-infrared fluorophores for mitochondrial imaging and single molecule spectroscopy

• Blaise Gatin-Fraudet; Julius Seifert; Kris Sarach; Christina Holmboe Olesen; Ramona Birke; Sigrid Milles; Melissa Birol; Martin Lehmann; Johannes Broichhagen

Chemical Communications 2025

Deuteration as a General Strategy to Enhance Azobenzene‐Based Photopharmacology

• Kilian Roßmann; Alberto J. Gonzalez‐Hernandez; Rahul Bhuyan; Caspar Schattenberg; Han Sun; Karl Börjesson; Joshua Levitz; Johannes Broichhagen

Angewandte Chemie International Edition 2024

Deuteration as a General Strategy to Enhance Azobenzene‐Based Photopharmacology

• Kilian Roßmann; Alberto J. Gonzalez‐Hernandez; Rahul Bhuyan; Caspar Schattenberg; Han Sun; Karl Börjesson; Joshua Levitz; Johannes Broichhagen

Angewandte Chemie 2024

Multicolor, Cell-Impermeable, and High Affinity BACE1 Inhibitor Probes Enable Superior Endogenous Staining and Imaging of Single Molecules

• Florian Stockinger; Pascal Poc; Alexander Möhwald; Sandra Karch; Stephanie Häfner; Christian Alzheimer; Guillaume Sandoz; Tobias Huth; Johannes Broichhagen

Journal of Medicinal Chemistry 2024

Direct optical measurement of intramolecular distances with angstrom precision

• Steffen J. Sahl; Jessica Matthias; Kaushik Inamdar; Michael Weber; Taukeer A. Khan; Christian Brüser; Stefan Jakobs; Stefan Becker; Christian Griesinger; Johannes Broichhagen; Stefan W. Hell

Science 2024

The MRAP2 accessory protein directly interacts with melanocortin-3 receptor to enhance signaling

• Aqfan Jamaluddin; Rachael A. Wyatt; Joon Lee; Georgina K.C. Dowsett; John A. Tadross; Johannes Broichhagen; Giles S.H. Yeo; Joshua Levitz; Caroline M. Gorvin

Melanocortin system activates carotid body arterial chemoreceptors in hypertension

• Audrys G. Pauza; Pratik Thakkar; Xin Shen; Igor Felippe; Kilian Roßmann; Manami Oya; Kazuhiro Nakamura; Johannes Broichhagen; David J. Hodson; Dainius H. Pauza; David Murphy; Julian F.R. Paton

Projection-Targeted Photopharmacology Reveals Distinct Anxiolytic Roles for Presynaptic mGluR2 in Prefrontal- and Insula-Amygdala Synapses

• Hermany Munguba; Vanessa A. Gutzeit; Ipsit Srivastava; Melanie Kristt; Ashna Singh; Akshara Vijay; Anisul Arefin; Sonal Thukral; Johannes Broichhagen; Joseph M. Stujenske; Conor Liston; Joshua Levitz

Fine‐tuned photochromic sulfonylureas for optical control of beta cell Ca2+ fluxes

• Ann‐Kathrin Rückert; Julia Ast; Annie Hasib; Daniela Nasteska; Katrina Viloria; Johannes Broichhagen; David J. Hodson

Diabetic Medicine 2023

Deuteration provides a general strategy to enhance azobenzene-based photopharmacology

• Kilian Roßmann; Alberto J. Gonzalez-Hernandez; Rahul Bhuyan; Karl Börjesson; Joshua Levitz; Johannes Broichhagen

Distinct beta-arrestin coupling and intracellular trafficking of metabotropic glutamate receptor homo- and heterodimers

• Joon Lee; Alberto J. Gonzalez-Hernandez; Melanie Kristt; Nohely Abreu; Kilian Roßmann; Anisul Arefin; Dagan C. Marx; Johannes Broichhagen; Joshua Levitz

Science Advances 2023

Real-Time Library Search Increases Cross-Link Identification Depth across All Levels of Sample Complexity

• Max Ruwolt; Yi He; Diogo Borges Lima; William Barshop; Johannes Broichhagen; Romain Huguet; Rosa Viner; Fan Liu

Analytical Chemistry 2023

Hypothalamic and brainstem glucose-dependent insulinotropic polypeptide receptor neurons employ distinct mechanisms to affect feeding

• Alice Adriaenssens; Johannes Broichhagen; Anne de Bray; Julia Ast; Annie Hasib; Ben Jones; Alejandra Tomas; Natalie Figueredo Burgos; Orla Woodward; Jo Lewis; Elisabeth O’Flaherty; Kimberley El; Canqi Cui; Norio Harada; Nobuya Inagaki; Jonathan Campbell; Daniel Brierley; David J. Hodson; Ricardo Samms; Fiona Gribble; Frank Reimann

JCI Insight 2023

Direct optical measurement of intra-molecular distances down to the Ångström scale

• Steffen J. Sahl; Jessica Matthias; Kaushik Inamdar; Taukeer A. Khan; Michael Weber; Stefan Becker; Christian Griesinger; Johannes Broichhagen; Stefan W. Hell

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

• Jerome Jatzlau; Wiktor Burdzinski; Michael Trumpp; Leon Obendorf; Kilian Roßmann; Katharina Ravn; Marko Hyvönen; Francesca Bottanelli; Johannes Broichhagen; Petra Knaus

Communications Biology 2023

Real-time library search increases cross-link identification depth across all levels of sample complexity

• Max Ruwolt; Yi He; Diogo Borges Lima; William Barshop; Johannes Broichhagen; Romain Huguet; Rosa Viner; Fan Liu

GLP1R Attenuates Sympathetic Response to High Glucose via Carotid Body Inhibition

• Audrys G. Pauza; Pratik Thakkar; Tatjana Tasic; Igor Felippe; Paul Bishop; Michael P. Greenwood; Kristina Rysevaite-Kyguoliene; Julia Ast; Johannes Broichhagen; David J. Hodson; Helio C. Salgado; Dainius H. Pauza; Nina Japundzic-Zigon; Julian F.R. Paton; David Murphy

Circulation Research 2022

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

• Jerome Jatzlau; Wiktor Burdzinski; Michael Trumpp; Leon Obendorf; Kilian Roßmann; Katharina Ravn; Marko Hyvönen; Francesca Bottanelli; Johannes Broichhagen; Petra Knaus

Restoration of high-sensitivity patterned vision in motion with an engineered light-gated G protein-coupled receptor

• Amy Holt; Michael H. Berry; Jamie Lo; Prashant Donthamsetti; Meike Visel; Johannes Broichhagen; John G. Flannery; Ehud Y. Isacoff

DFT‐basierte Entdeckung von Ethynyl‐Triazolyl‐Phosphinaten als modulare Elektrophile für die chemoselektive Cystein‐Biokonjugation und Profilierung

• Christian E. Stieger; Yerin Park; Mark A. R. de Geus; Dongju Kim; Christiane Huhn; J. Sophia Slenczka; Philipp Ochtrop; Judith M. Müchler; Roderich D. Süssmuth; Johannes Broichhagen; Mu‐Hyun Baik; Christian P. R. Hackenberger

Angewandte Chemie 2022

DFT‐Guided Discovery of Ethynyl‐Triazolyl‐Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling

• Christian E. Stieger; Yerin Park; Mark A. R. de Geus; Dongju Kim; Christiane Huhn; J. Sophia Slenczka; Philipp Ochtrop; Judith M. Müchler; Roderich D. Süssmuth; Johannes Broichhagen; Mu‐Hyun Baik; Christian P. R. Hackenberger

Angewandte Chemie International Edition 2022

Back Cover: DFT‐Guided Discovery of Ethynyl‐Triazolyl‐Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling (Angew. Chem. Int. Ed. 41/2022)

• Christian E. Stieger; Yerin Park; Mark A. R. de Geus; Dongju Kim; Christiane Huhn; J. Sophia Slenczka; Philipp Ochtrop; Judith M. Müchler; Roderich D. Süssmuth; Johannes Broichhagen; Mu‐Hyun Baik; Christian P. R. Hackenberger

Angewandte Chemie International Edition 2022

Rücktitelbild: DFT‐basierte Entdeckung von Ethynyl‐Triazolyl‐Phosphinaten als modulare Elektrophile für die chemoselektive Cystein‐Biokonjugation und Profilierung (Angew. Chem. 41/2022)

• Christian E. Stieger; Yerin Park; Mark A. R. de Geus; Dongju Kim; Christiane Huhn; J. Sophia Slenczka; Philipp Ochtrop; Judith M. Müchler; Roderich D. Süssmuth; Johannes Broichhagen; Mu‐Hyun Baik; Christian P. R. Hackenberger

Angewandte Chemie 2022

Control of Gα q signaling dynamics and GPCR cross-talk by GRKs

• Guoqing Xiang; Amanda Acosta-Ruiz; Arthur Radoux-Mergault; Melanie Kristt; Jihye Kim; Jared D. Moon; Johannes Broichhagen; Asuka Inoue; Francis S. Lee; Miriam Stoeber; Jeremy S. Dittman; Joshua Levitz

Science Advances 2022

An examination of the divergent spatiotemporal signaling of GLP-1R versus GIPR in pancreatic beta cells

• Yusman Manchanda; Stavroula Bitsi; Shiqian Chen; Johannes Broichhagen; Jorge Bernardino de la Serna; Ben Jones; Alejandra Tomas

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

• Michael Trumpp; Anna Oliveras; Hannes Gonschior; Julia Ast; David J. Hodson; Petra Knaus; Martin Lehmann; Melissa Birol; Johannes Broichhagen

Chemical Communications 2022

Advances in tethered photopharmacology for precise optical control of signaling proteins

• Johannes Broichhagen; Joshua Levitz

Current Opinion in Pharmacology 2022

Visualisierung endogener Proteine in ihrer natürlichen Umgebung

• Christiane Huhn; Johannes Broichhagen

BIOspektrum 2022

Structural and compositional diversity in the kainate receptor family

• Purushotham Selvakumar; Joon Lee; Nandish Khanra; Changhao He; Hermany Munguba; Lisa Kiese; Johannes Broichhagen; Andreas Reiner; Joshua Levitz; Joel R. Meyerson

Cell Reports 2021

Reagents and models for detecting endogenous GLP1R and GIPR

• Julia Ast; Johannes Broichhagen; David J. Hodson

eBioMedicine 2021

Chemical Biology Tools To Investigate Malaria Parasites

• Johannes Broichhagen; Nicole Kilian

ChemBioChem 2021

Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors

• Jordana K. Thibado; Jean-Yves Tano; Joon Lee; Leslie Salas-Estrada; Davide Provasi; Alexa Strauss; Joao Marcelo Lamim Ribeiro; Guoqing Xiang; Johannes Broichhagen; Marta Filizola; Martin Lohse; Joshua Levitz

Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking

• Maria Lucey; Tanyel Ashik; Amaara Marzook; Yifan Wang; Joëlle Goulding; Atsuro Oishi; Johannes Broichhagen; David J Hodson; James Minnion; Yuval Elani; Ralf Jockers; Stephen J Briddon; Stephen R Bloom; Alejandra Tomas; Ben Jones

Evaluation of efficacy-versusaffinity-driven agonism with biased GLP-1R ligands P5 and exendin-F1

• Amaara Marzook; Shiqian Chen; Phil Pickford; Maria Lucey; Yifan Wang; Ivan R Corrêa, Jr; Johannes Broichhagen; David J Hodson; Victoria Salem; Guy A Rutter; Tricia M Tan; Stephen R Bloom; Alejandra Tomas; Ben Jones

Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors

• Jordana K Thibado; Jean-Yves Tano; Joon Lee; Leslie Salas-Estrada; Davide Provasi; Alexa Strauss; Joao Marcelo Lamim Ribeiro; Guoqing Xiang; Johannes Broichhagen; Marta Filizola; Martin J Lohse; Joshua Levitz

eLife 2021

Sequentialin vivolabeling of insulin secretory granule pools in INS-SNAP transgenic pigs

• Elisabeth Kemter; Andreas Müller; Martin Neukam; Anna Ivanova; Nikolai Klymiuk; Simone Renner; Kaiyuan Yang; Johannes Broichhagen; Mayuko Kurome; Valerie Zakhartchenko; Barbara Kessler; Klaus-Peter Knoch; Marc Bickle; Barbara Ludwig; Kai Johnsson; Heiko Lickert; Thomas Kurth; Eckhard Wolf; Michele Solimena

SNAP-tag-enabled super-resolution imaging reveals constitutive and agonist-dependent trafficking of GPR56 in pancreatic β-cells

• Oladapo E. Olaniru; Jordan Cheng; Julia Ast; Anastasia Arvaniti; Patricio Atanes; Guo C. Huang; Aileen J.F. King; Peter M. Jones; Johannes Broichhagen; David J. Hodson; Shanta J. Persaud

Molecular Metabolism 2021

Sequential in vivo labeling of insulin secretory granule pools in INS - SNAP transgenic pigs

• Elisabeth Kemter; Andreas Müller; Martin Neukam; Anna Ivanova; Nikolai Klymiuk; Simone Renner; Kaiyuan Yang; Johannes Broichhagen; Mayuko Kurome; Valeri Zakhartchenko; Barbara Kessler; Klaus-Peter Knoch; Marc Bickle; Barbara Ludwig; Kai Johnsson; Heiko Lickert; Thomas Kurth; Eckhard Wolf; Michele Solimena

Proceedings of the National Academy of Sciences 2021

Purification of age-distinct insulin secretory granules through antigen restriction

• Martin Neukam; Pia Sala; Andreas-David Brunner; Katharina Ganß; Alessandra Palladini; Michal Grzybek; Jovana Vasiljević; Johannes Broichhagen; Kai Johnsson; Thomas Kurth; Matthias Mann; Ünal Coskun; Michele Solimena

Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates

• Pascal Poc; Vanessa A. Gutzeit; Julia Ast; Joon Lee; Ben J. Jones; Elisa D’Este; Bettina Mathes; David J. Hodson; Joshua Levitz; Johannes Broichhagen

Transformation of Receptor Tyrosine Kinases into Glutamate Receptors and Photoreceptors

• Philipp Leippe; Johannes Broichhagen; Katia Cailliau; Alexandra Mougel; Marion Morel; Colette Dissous; Dirk Trauner; Jérôme Vicogne

Angewandte Chemie International Edition 2020

Transformation of Receptor Tyrosine Kinases into Glutamate Receptors and Photoreceptors

• Philipp Leippe; Johannes Broichhagen; Katia Cailliau; Alexandra Mougel; Marion Morel; Colette Dissous; Dirk Trauner; Jérôme Vicogne

Angewandte Chemie 2020

Deuterated rhodamines for protein labelling in nanoscopy

• Kilian Roßmann; Kerem C. Akkaya; Corentin Charbonnier; Jenny Eichhorst; Ben Jones; Martin Lehmann; Johannes Broichhagen

Photohormones Enable Optical Control of the Peroxisome Proliferator-Activated Receptor γ (PPARγ)

• Konstantin Hinnah; Sabine Willems; Johannes Morstein; Jan Heering; Felix W. W. Hartrampf; Johannes Broichhagen; Philipp Leippe; Daniel Merk; Dirk Trauner

Journal of Medicinal Chemistry 2020

Ligand-Specific Factors Influencing GLP-1 Receptor Post-Endocytic Trafficking and Degradation in Pancreatic Beta Cells

• Zijian Fang; Shiqian Chen; Yusman Manchanda; Stavroula Bitsi; Philip Pickford; Alessia David; Maria M. Shchepinova; Ivan R. Corrêa Jr; David J. Hodson; Johannes Broichhagen; Edward W. Tate; Frank Reimann; Victoria Salem; Guy A. Rutter; Tricia Tan; Stephen R. Bloom; Alejandra Tomas; Ben Jones

International Journal of Molecular Sciences 2020

Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin

• George Khelashvili; Anoop Narayana Pillai; Joon Lee; Kalpana Pandey; Alexander M. Payne; Zarek Siegel; Michel A. Cuendet; Tylor R. Lewis; Vadim Y. Arshavsky; Johannes Broichhagen; Joshua Levitz; Anant K. Menon

Optical Control of a Biological Reaction–Diffusion System

• Philipp Glock; Johannes Broichhagen; Simon Kretschmer; Philipp Blumhardt; Jonas Mücksch; Dirk Trauner; Petra Schwille

Angewandte Chemie 2018

Author Correction: Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor

• Michael H. Berry; Amy Holt; Joshua Levitz; Johannes Broichhagen; Benjamin M. Gaub; Meike Visel; Cherise Stanley; Krishan Aghi; Yang Joon Kim; Kevin Cao; Richard H Kramer; Dirk Trauner; John Flannery; Ehud Y. Isacoff

Nature Communications 2018

Conditional and Reversible Activation of Class A and B G Protein-Coupled Receptors Using Tethered Pharmacology

• Tom Podewin; Julia Ast; Johannes Broichhagen; Nicholas H. F. Fine; Daniela Nasteska; Philipp Leippe; Manuel Gailer; Teresa Buenaventura; Nisha Kanda; Ben J. Jones; Celine M’Kadmi; Jean-Louis Baneres; Jacky Marie; Alejandra Tomas; Dirk Trauner; Anja Hoffmann-Röder; David J. Hodson

ACS Central Science 2018

Optical Control of a Biological Reaction–Diffusion System

• Philipp Glock; Johannes Broichhagen; Simon Kretschmer; Philipp Blumhardt; Jonas Mücksch; Dirk Trauner; Petra Schwille

Angewandte Chemie International Edition 2018

A Predictive Approach for the Optical Control of Carbonic Anhydrase II Activity

• Kateri H. DuBay; Katharina Iwan; Laura Osorio-Planes; Phillip L. Geissler; Michael Groll; Dirk Trauner; Johannes Broichhagen

ACS Chemical Biology 2018

A (+)‐Larixol Congener with High Affinity and Subtype Selectivity toward TRPC6

• Stephanie Häfner; Finn Burg; Martina Kannler; Nicole Urban; Peter Mayer; Alexander Dietrich; Dirk Trauner; Johannes Broichhagen; Michael Schaefer

ChemMedChem 2018

A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1

• Sandra Karch; Johannes Broichhagen; Julia Schneider; Daniel Böning; Stephanie Hartmann; Benjamin Schmid; Philipp Tripal; Ralf Palmisano; Christian Alzheimer; Kai Johnsson; Tobias Huth

Journal of Medicinal Chemistry 2018

SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand

• Helen Farrants; Vanessa A. Gutzeit; Amanda Acosta-Ruiz; Dirk Trauner; Kai Johnsson; Joshua Levitz; Johannes Broichhagen

ACS Chemical Biology 2018

Optical tools for understanding the complexity of β-cell signalling and insulin release

• James A. Frank; Johannes Broichhagen; Dmytro A. Yushchenko; Dirk Trauner; Carsten Schultz; David J. Hodson

Nature Reviews Endocrinology 2018

Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor

• Michael H. Berry; Amy Holt; Joshua Levitz; Johannes Broichhagen; Benjamin M. Gaub; Meike Visel; Cherise Stanley; Krishan Aghi; Yang Joon Kim; Kevin Cao; Richard H. Kramer; Dirk Trauner; John Flannery; Ehud Y. Isacoff

Nature Communications 2017

Remote control of glucose homeostasis in vivo using photopharmacology

• Zenobia B. Mehta; Natalie R. Johnston; Marie-Sophie Nguyen-Tu; Johannes Broichhagen; Peter Schultz; Dean P. Larner; Isabelle Leclerc; Dirk Trauner; Guy A. Rutter; David J. Hodson

Scientific Reports 2017

Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors

• Joshua Levitz; Johannes Broichhagen; Philipp Leippe; David Konrad; Dirk Trauner; Ehud Y. Isacoff

Proceedings of the National Academy of Sciences 2017

Optical control of GPR40 signalling in pancreatic β-cells

• James Allen Frank; Dmytro A. Yushchenko; Nicholas H. F. Fine; Margherita Duca; Mevlut Citir; Johannes Broichhagen; David J. Hodson; Carsten Schultz; Dirk Trauner

Chem. Sci. 2017

Potent Prearranged Positive Allosteric Modulators of the Glucagon‐like Peptide‐1 Receptor

• Ben J. Jones; Rosario Scopelliti; Alejandra Tomas; Stephen R. Bloom; David J. Hodson; Johannes Broichhagen

ChemistryOpen 2017

Optical control of a receptor-linked guanylyl cyclase using a photoswitchable peptidic hormone

• Tom Podewin; Johannes Broichhagen; Christina Frost; Dieter Groneberg; Julia Ast; Helena Meyer-Berg; Nicholas H. F. Fine; Andreas Friebe; Martin Zacharias; David J. Hodson; Dirk Trauner; Anja Hoffmann-Röder

Chemical Science 2017

Azobenzene-based inhibitors of human carbonic anhydrase II.

• Runtsch LS; Barber DM; Mayer P; Groll M; Trauner D; Broichhagen J

A roadmap to success in photopharmacology.

• Broichhagen J; Frank JA; Trauner D

Crystal structure of (E)-1,2-bis-(4-bromo-2,6-di-fluoro-phen-yl)diazene.

• Broichhagen J; Woodmansee DH; Trauner D; Mayer P

AzoCholine Enables Optical Control of Alpha 7 Nicotinic Acetylcholine Receptors in Neural Networks.

• Damijonaitis A; Broichhagen J; Urushima T; Hüll K; Nagpal J; Laprell L; Schönberger M; Woodmansee DH; Rafiq A; Sumser MP; Kummer W; Gottschalk A; Trauner D

A red-shifted photochromic sulfonylurea for the remote control of pancreatic beta cell function.

• Broichhagen J; Frank JA; Johnston NR; Mitchell RK; Šmid K; Marchetti P; Bugliani M; Rutter GA; Trauner D; Hodson DJ

Optical Control of Insulin Secretion Using an Incretin Switch.

• Broichhagen J; Podewin T; Meyer-Berg H; von Ohlen Y; Johnston NR; Jones BJ; Bloom SR; Rutter GA; Hoffmann-Röder A; Hodson DJ; Trauner D