TechnologieplattformMolekulare Physiologie & Zellbiologie

Ralf Schülein

Cell Engineering

portrait ralf schuelein

Die Cell Engineering Facility setzt modernste CRISPR/Cas-Techniken ein, um Gen-Knock-outs und Knock-ins in Zielzellen zu erzeugen. Andere Anwendungen von CRISPR/Cas sind nach Rücksprache möglich.


Die Cell Engineering Facility setzt CRISPR/Cas-Techniken ein, um Gen-Knock-Outs und Knock-Ins in Zielzellen zu erzeugen. Mit Hilfe der entstandenen Zellklone kann die Funktion spezifischer Gene und Proteine ​​im zellulären Kontext detailliert untersucht werden. Gen-Knock-Out bedeutet, dass die Funktion eines bestimmten Gens und folglich die seines kodierten Proteins durch eine eingeführte Mutation zerstört werden. Gen-Knock-In bedeutet, dass das spezifische Zielgen und sein Protein aufgrund der eingeführten Mutation eine neue Funktion erhalten.


General Information

After its publication in 2012, the CRISPR/Cas gene editing system revolutionized molecular biology within only a few years (CRISPR = clustered regularly interspaced short palindromic repeats; see also our Introduction page). The increase in publications using CRISPR/Cas is only comparable to what was seen in the eighties following the development of the polymerase chain reaction (PCR) or in the nineties following the introduction of the green fluorescent protein (GFP). The Cell Engineering Facility of the FMP will make gene knock-outs and knock-ins in various target cells using state-of-the art CRISPR/Cas techniques. Other applications may be possible following consultation. Cell clones will be characterized by DNA sequencing and the genotype will be analyzed to confirm modification of both alleles. Western blotting will be done in case you could provide a specific antibody.
An overview of the methodology we use can be found on the “Methods” page. If you are already familiar with CRISPR/Cas, you may immediately proceed to the “Order” page. At the moment, we will be able to make 2 clones for each FMP group/year. Depending on the amount of simultaneous orders, clones should be ready within 8-12 weeks. If you use the system for the first time or need an overview of its history, function and applications, you may visit our “Introduction” page first. If you have any questions, please get in touch.
Depending on our expenses and the amount of sgRNAs needed, the costs for a cell clone will be in range of 1,500 to 2,500€ and will be charged once the clones are ready.

In addition to CRISPR/Cas gene editing, the Cell Engineering Facility is also able to make stable transfected cell clones using various cell lines upon request. 

Introduction into CRISPR/Cas

Publikationen (ORCID)

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The Pseudo signal peptide of the corticotropin-releasing factor receptor type 2A prevents receptor oligomerization

  • Teichmann, Anke; Rutz, Claudia; Kreuchwig, Annika; Krause, Gerd; Wiesner, Burkhard; Schülein, Ralf

The Journal of biological chemistry 2012

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The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression

  • Zampatis, Dimitris E; Rutz, Claudia; Furkert, Jens; Schmidt, Antje; Wüstenhagen, Doreen; Kubick, Stefan; Tsopanoglou, Nikos E; Schülein, Ralf

FEBS letters 2012

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Live cell imaging of G protein-coupled receptors

  • Teichmann, Anke; Schmidt, Antje; Wiesner, Burkhard; Oksche, Alexander; Schülein, Ralf

Methods in molecular biology (Clifton, N.J.) 2012

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Use of Kikume green-red fusions to study the influence of pharmacological chaperones on trafficking of G protein-coupled receptors

  • Ridelis, Ingrid; Schmidt, Antje; Teichmann, Anke; Furkert, Jens; Wiesner, Burkhard; Schülein, Ralf

FEBS letters 2012

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Functional significance of cleavable signal peptides of G protein-coupled receptors

  • Schülein, Ralf; Westendorf, Carolin; Krause, Gerd; Rosenthal, Walter

European journal of cell biology 2012

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The proteoglycan syndecan 4 regulates transient receptor potential canonical 6 channels via RhoA/Rho-associated protein kinase signaling

  • Liu, Ying; Echtermeyer, Frank; Thilo, Florian; Theilmeier, Gregor; Schmidt, Antje; Schülein, Ralf; Jensen, Boye L; Loddenkemper, Christoph; Jankowski, Vera; Marcussen, Niels; Gollasch, Maik; Arendshorst, William J; Tepel, Martin

Arteriosclerosis, thrombosis, and vascular biology 2012

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