Jens Peter von Kries

Scientific Focus

[Translate to Deutsch:] drawing by virchow

The new old-fashioned way: Virchow 2.0
Virchow’s teachings continue to this day – using full computing power

Mission
Open access for academic projects and SME´s towards experiments in high-throughput is our major aim.

Highlights
Find some examples of our Research Highlights

Chemical Biology
Specific Features of Small Molecules used for Biology

Open Access Plattform

Project Application

You may contact us via < screening@fmp-berlin.de >

For effective support and data documentation you need to fill in the requested information and send us the project application form

Assay Setup

You may visit the Screening Unit, where we can help you in assay transfer to the 384well format and test your assay set up together for HTS usage. You will then be able to update the project application form with the revised protocol. Afterwards the materials for your next visit and primary screen plus validation are calculated.

Primary Screen

We perform the primary screen together with one person from your team, the data are documented and analyzed automatically. You receive the raw data, statistic analysis, and statistics-based hit lists containing also structural data.

Review of hit lists

If desired, you may revise the hit lists. You send us a list of 352 compounds via e-mail.

Cherry picking

We provide you with a 384-well plate containing your requested 352 hits, (4 µl 5mM compound in DMSO).

Quality control of 352 hit compounds via LC-MS Analysis

A small aliquot (0.2 µl) of your hit picking plate is taken and diluted in ACN/H₂O for the LC-MS analysis. We analyze the samples in ACN/H₂O (1:1, 25 µM) with our Agilent TOF mass spectrometer and determine the purity via UV absorption at 254 nm.

IC₅₀ validation

IC₅₀validation (using nine serial dilutions of the compounds in duplicate) is carried out by us, the data are analyzed automatically. You receive for a maximum of 352 "hit" compounds IC₅₀ reports containing MS analysis data, frequent hitter information, data from biophysical and cytotox profiling. To our experience less then 10% of the compounds from the hit list of 352 can be expected to be true hits.

Review of IC₅₀data

Our chemists revise with you the list of validated compounds and suggest proposals, which compounds might be selected for further characterization and optimization of leads.

Please bear in mind that all steps need only to be performed once per project!

The Screening Unit can assist your project with know-how gained from about 170 previous screens. But the data documentation, analysis and follow up studies are in the responsibility of the PI of the project.

RNAi Screening

The facility offers scientists from the FMP and MDC access to high-throughput screening with arrayed siRNA libraries on the basis of a scientific collaboration.

Broad range of assays
State of the art equipment supports a broad range of assays in high-throughput formats, ranging from basic reporter assays to high-content screening campaigns. Instrumentation covers automated fluorescence microscopy, luminescence and real-time kinetic fluorescence reading.

Full support in high-throughput screening
We operate on the basis of a scientific collaboration and offer support through all stages of a screening campaign: assay development and adaption to high-throughput screening, the actual wet-lab screen, data acquisition and quality assessment, statistical analysis as well as hit candidate selection.

Data processing
We are constantly advancing our in-house analysis, database and reporting tools to keep the platform updated. The integration of freely available open-source tools (e.g. KNIME Analytics Platform) and the development of our own analysis workflows enables us to meet the specific requirements of a multitude of biological questions.

Surface Plasmon Resonance

The Surface Plasmon Resonance detection system allows automated screening for compounds (even 100 Dalton sized) which bind to proteins immobilized on chips. Different types of chip surfaces allow to couple proteins under mild conditions via His- or GSH- tags, by antibodies or strepravidine.
Specific biological activity of proteins must be controlled by defined binders and non-binders to monitor stability of proteins after repeated regeneration steps for removal of nonspecifically binding compounds.
Kd, plus On and Off rates can be calculated from kinetic data.

The service can be used to characterize small molecules that emerged as hit compounds from a screening project, but also user-provided compounds can be tested.

Technologien

Detection Techniques

Automated Data Documentation & Analysis

Genomic Screening Techniques

Liquid handling systems

Tools for Assay Setup

Tools

Publikationen

Selected Projects and Publications
Find a List of selected Projects and Literature

List of Publications
Overview of Publications

Kontakt

Dr. Jens Peter von Kries

Leitung, Screening-Unit

+49 30 94793 551
screening@fmp-berlin.de

Kontakt Sekretariat

Dajana Baudach

Sekretariat, Sun Gruppe,
Sekretariat Nazaré Gruppe

+49 3094793 570
baudach@fmp-berlin.de

Forschungsbereich

Chemische Biologie

Publikationen via ORCID

Publikationen

2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2000 1996 1995 1994 1991 1990 1988

Structure-Based Design of Xanthine-Imidazopyridines and -Imidazothiazoles as Highly Potent and In Vivo Efficacious Tryptophan Hydroxylase Inhibitors.

Specker E; Wesolowski R; Schütz A; Matthes S; Mallow K; Wasinska-Kalwa M; Winkler L; Oder A; Alenina N; Pleimes D; von Kries JP; Heinemann U; Bader M; Nazaré M

Journal of medicinal chemistry 2023

online lesen

Discovery of tetrazolo-pyridazine-based small molecules as inhibitors of MACC1-driven cancer metastasis.

Yan S; Schöpe PC; Lewis J; Putzker K; Uhrig U; Specker E; von Kries JP; Lindemann P; Omran A; Sanchez-Ibarra HE; Unger A; Zischinsky ML; Klebl B; Stein U

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023

online lesen

An unconventional gatekeeper mutation sensitizes inositol hexakisphosphate kinases to an allosteric inhibitor.

Aguirre T; Dornan GL; Hostachy S; Neuenschwander M; Seyffarth C; Haucke V; Schütz A; von Kries JP; Fiedler D

eLife 2023

online lesen

Small Molecules Targeting Human UDP-GlcNAc 2-Epimerase.

Gorenflos López JL; Dornan GL; Boback N; Neuenschwander M; Oder A; Kemnitz-Hassanin K; Schmieder P; Specker E; Asikoglu HC; Oberdanner C; Hackenberger CPR

Chembiochem : a European journal of chemical biology 2023

online lesen

Structural Basis for Highly Selective Class II Alpha Phosphoinositide-3-Kinase Inhibition.

Kücükdisli M; Bel-Abed H; Cirillo D; Lo WT; Efrém NL; Horatscheck A; Perepelittchenko L; Prokofeva P; Ehret TAL; Radetzki S; Neuenschwander M; Nazaré M

Journal of medicinal chemistry 2023

online lesen

7,8-Dihydroxyflavone is a direct inhibitor of pyridoxal phosphatase

Zink C; Witzinger L; Keller A; Hadamek K; Bothe S; Neuenschwander M; Villmann C; von Kries JP; Schindelin H; Jeanclos E; Gohla A

online lesen

Role of endothelial cells and angiotensin converting enzyme-II in COVID-19 and brain damages post-infection.

Mehboob R; von Kries JP; Ehsan K; Almansouri M; Bamaga AK

Frontiers in neurology 2023

online lesen

An unconventional gatekeeper mutation sensitizes inositol hexakisphosphate kinases to an allosteric inhibitor

Aguirre T; Dornan GL; Hostachy S; Neuenschwander M; Seyffarth C; Haucke V; Schütz A; von Kries JP; Fiedler D

online lesen

Large-scale microRNA functional high-throughput screening identifies miR-515-3p and miR-519e-3p as inducers of human cardiomyocyte proliferation.

Renikunta HV; Lazarow K; Gong Y; Shukla PC; Nageswaran V; Giral H; Kratzer A; Opitz L; Engel FB; Haghikia A; Costantino S; Paneni F; von Kries JP; Jakob P

iScience 2023

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Small molecule inhibiting microglial nitric oxide release could become a potential treatment for neuroinflammation.

Jordan P; Costa A; Specker E; Popp O; Volkamer A; Piske R; Obrusnik T; Kleissle S; Stuke K; Rex A; Neuenschwander M; von Kries JP; Nazare M; Wolf SA

PloS one 2023

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The FGFR inhibitor PD173074 binds to the C-terminus of oncofetal HMGA2 and modulates its DNA-binding and transcriptional activation functions.

Ahmed SM; Ragunathan P; Shin J; Peter S; Kleissle S; Neuenschwander M; Schäfer R; Kries JPV; Grüber G; Dröge P

FEBS letters 2023

online lesen

Glycolytic flux control by drugging phosphoglycolate phosphatase.

Jeanclos E; Schlötzer J; Hadamek K; Yuan-Chen N; Alwahsh M; Hollmann R; Fratz S; Yesilyurt-Gerhards D; Frankenbach T; Engelmann D; Keller A; Gohla A

Nature communications 2022

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TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-κB activation.

Tufan AB; Lazarow K; Kolesnichenko M; Sporbert A; von Kries JP; Scheidereit C

The EMBO journal 2022

online lesen

Development of selective inhibitors of phosphatidylinositol 3-kinase C2α.

Lo WT; Belabed H; Kücükdisli M; Metag J; Roske Y; Prokofeva P; Ohashi Y; Horatscheck A; Cirillo D; Krauss M; Schmied C; Neuenschwander M; Haucke V

Nature chemical biology 2022

online lesen

Structure-Based Design of Xanthine-Benzimidazole Derivatives as Novel and Potent Tryptophan Hydroxylase Inhibitors.

Specker E; Matthes S; Wesolowski R; Schütz A; Grohmann M; Alenina N; Pleimes D; Mallow K; Neuenschwander M; Gogolin A; Weise M; Pfeifer J; Ziebart N; Bader M

Journal of medicinal chemistry 2022

online lesen

Disruptors of AKAP-Dependent Protein-Protein Interactions.

Walker-Gray R; Pallien T; Miller DC; Oder A; Neuenschwander M; von Kries JP; Diecke S; Klussmann E

Methods in molecular biology (Clifton, N.J.) 2022

online lesen

Aurora Kinase A Is Involved in Controlling the Localization of Aquaporin-2 in Renal Principal Cells

Sandrine Baltzer; Timur Bulatov; Christopher Schmied; Andreas Krämer; Benedict-Tilman Berger; Andreas Oder; Ryan Walker-Gray; Christin Kuschke; Kerstin Zühlke; Jenny Eichhorst; Martin Lehmann; Stefan Knapp; John Weston; von Kries, J.P.; Roderich D. Süssmuth; Enno Klussmann

International Journal of Molecular Sciences 2022

online lesen

TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB Activation

Tufan AB; Lazarow K; Kolesnichenko M; Sporbert A; von Kries JP; Scheidereit C

online lesen

Proteasome inhibitors suppress MYB oncogenic activity in a p300-dependent manner.

Yusenko MV; Biyanee A; Andersson MK; Radetzki S; von Kries JP; Stenman G; Klempnauer KH

Cancer letters 2021

online lesen

Author Correction: Loss-of-function uORF mutations in human malignancies.

Schulz J; Mah N; Neuenschwander M; Kischka T; Ratei R; Schlag PM; Castaños-Vélez E; Fichtner I; Tunn PU; Denkert C; Klaas O; Berdel WE; von Kries JP; Wethmar K

Scientific reports 2021

online lesen

A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation.

Ostrop J; Zwiggelaar RT; Terndrup Pedersen M; Gerbe F; Bösl K; Lindholm HT; Díez-Sánchez A; Parmar N; Radetzki S; von Kries JP; Jay P; Jensen KB; Oudhoff MJ

Frontiers in cell and developmental biology 2021

online lesen

Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis.

Bonnekoh H; Vera C; Abad-Perez A; Radetzki S; Neuenschwander M; Specker E; Mahnke NA; Frischbutter S; Latz E; Nazaré M; Kries JV; Maurer M; Scheffel J; Krause K

Clinical and translational allergy 2021

online lesen

From Pyrazolones to Azaindoles: Evolution of Active-Site SHP2 Inhibitors Based on Scaffold Hopping and Bioisosteric Replacement.

Mostinski Y; Heynen GJJE; López-Alberca MP; Paul J; Miksche S; Radetzki S; Schaller D; Shanina E; Seyffarth C; Kolomeets Y; Ziebart N; de Schryver J; Nazaré M

Journal of medicinal chemistry 2020

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A semi-automated organoid screening method demonstrates epigenetic control of intestinal epithelial differentiation

Ostrop J; Zwiggelaar R; Pedersen MT; Gerbe F; Bösl K; Lindholm HT; Díez-Sánchez A; Parmar N; Radetzki S; von Kries JP; Jay P; Jensen KB; Arrowsmith C; Oudhoff MJ

online lesen

Cyclin-Dependent Kinase 18 Controls Trafficking of Aquaporin-2 and Its Abundance through Ubiquitin Ligase STUB1, Which Functions as an AKAP.

Dema A; Faust D; Lazarow K; Wippich M; Neuenschwander M; Zühlke K; Geelhaar A; Pallien T; Hallscheidt E; Eichhorst J; Wiesner B; Černecká H; Popp O; Klussmann E

Cells 2020

online lesen

Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels.

Ullrich F; Blin S; Lazarow K; Daubitz T; von Kries JP; Jentsch TJ

eLife 2019

online lesen

Regulation of influenza A virus mRNA splicing by CLK1.

Artarini A; Meyer M; Shin YJ; Huber K; Hilz N; Bracher F; Eros D; Orfi L; Keri G; Goedert S; Neuenschwander M; von Kries J; Domovich-Eisenberg Y; Karlas A

Antiviral research 2019

online lesen

Vinylphosphonites for Staudinger-induced chemoselective peptide cyclization and functionalization.

Kasper MA; Glanz M; Oder A; Schmieder P; von Kries JP; Hackenberger CPR

Chemical science 2019

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High-Throughput Screening for Modulators of CFTR Activity Based on Genetically Engineered Cystic Fibrosis Disease-Specific iPSCs.

Merkert S; Schubert M; Olmer R; Engels L; Radetzki S; Veltman M; Scholte BJ; Zöllner J; Pedemonte N; Galietta LJV; von Kries JP; Martin U

Stem cell reports 2019

online lesen

EU-OPENSCREEN: A Novel Collaborative Approach to Facilitate Chemical Biology.

Brennecke P; Rasina D; Aubi O; Herzog K; Landskron J; Cautain B; Vicente F; Quintana J; Mestres J; Stechmann B; Ellinger B; Brea J; Kolanowski JL; Gribbon P

SLAS discovery : advancing life sciences R & D 2019

online lesen

Identification of TMEM206 proteins as pore of ASOR acid-sensitive chloride channels

Ullrich F; Blin S; Lazarow K; Daubitz T; Kries Jv; Jentsch TJ

online lesen

A New Highly Thyrotropin Receptor-Selective Small-Molecule Antagonist with Potential for the Treatment of Graves' Orbitopathy.

Marcinkowski P; Hoyer I; Specker E; Furkert J; Rutz C; Neuenschwander M; Sobottka S; Sun H; Nazare M; Berchner-Pfannschmidt U; von Kries JP; Krause G

Thyroid : official journal of the American Thyroid Association 2018

online lesen

Use of a sequential high throughput screening assay to identify novel inhibitors of the eukaryotic SRP-Sec61 targeting/translocation pathway.

Klein W; Rutz C; Eckhard J; Provinciael B; Specker E; Neuenschwander M; Kleinau G; Scheerer P; von Kries JP; Nazaré M; Vermeire K; Schülein R

PloS one 2018

online lesen

Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations.

Otten C; Knox J; Boulday G; Eymery M; Haniszewski M; Neuenschwander M; Radetzki S; Vogt I; Hähn K; De Luca C; Cardoso C; Hamad S; Igual Gil C; Roy P; Abdelilah-Seyfried S

EMBO molecular medicine 2018

online lesen

Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling.

Liss M; Radke MH; Eckhard J; Neuenschwander M; Dauksaite V; von Kries JP; Gotthardt M

PloS one 2018

online lesen

Loss-of-function uORF mutations in human malignancies.

Schulz J; Mah N; Neuenschwander M; Kischka T; Ratei R; Schlag PM; Castaños-Vélez E; Fichtner I; Tunn PU; Denkert C; Klaas O; Berdel WE; von Kries JP; Wethmar K

Scientific reports 2018

online lesen

An AKAP-Lbc-RhoA interaction inhibitor promotes the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells.

Schrade K; Tröger J; Eldahshan A; Zühlke K; Abdul Azeez KR; Elkins JM; Neuenschwander M; Oder A; Elkewedi M; Jaksch S; Andrae K; Li J; Fernandes J; Klussmann E

PloS one 2018

online lesen

Small Molecules Targeting Human N-Acetylmannosamine Kinase.

Hinderlich S; Neuenschwander M; Wratil PR; Oder A; Lisurek M; Nguyen LD; von Kries JP; Hackenberger CPR

Chembiochem : a European journal of chemical biology 2017

online lesen

Discovery of a Novel Series of Tankyrase Inhibitors by a Hybridization Approach.

Anumala UR; Waaler J; Nkizinkiko Y; Ignatev A; Lazarow K; Lindemann P; Olsen PA; Murthy S; Obaji E; Majouga AG; Leonov S; von Kries JP; Lehtiö L; Nazaré M

Journal of medicinal chemistry 2017

online lesen

A Chemical Disruptor of the ClpX Chaperone Complex Attenuates the Virulence of Multidrug-Resistant Staphylococcus aureus.

Fetzer C; Korotkov VS; Thänert R; Lee KM; Neuenschwander M; von Kries JP; Medina E; Sieber SA

Angewandte Chemie (International ed. in English) 2017

online lesen

Neuronal Chemosensation and Osmotic Stress Response Converge in the Regulation of <i>aqp-8</i> in <i>C. elegans</i>.

Igual Gil C; Jarius M; von Kries JP; Rohlfing AK

Frontiers in physiology 2017

online lesen

Statin and rottlerin small-molecule inhibitors restrict colon cancer progression and metastasis via MACC1.

Juneja M; Kobelt D; Walther W; Voss C; Smith J; Specker E; Neuenschwander M; Gohlke BO; Dahlmann M; Radetzki S; Preissner R; von Kries JP; Schlag PM; Stein U

PLoS biology 2017

online lesen

Identification of a Novel Benzimidazole Pyrazolone Scaffold That Inhibits KDM4 Lysine Demethylases and Reduces Proliferation of Prostate Cancer Cells.

Carter DM; Specker E; Przygodda J; Neuenschwander M; von Kries JP; Heinemann U; Nazaré M; Gohlke U

SLAS discovery : advancing life sciences R & D 2017

online lesen

Small-molecule inhibition of STOML3 oligomerization reverses pathological mechanical hypersensitivity.

Wetzel C; Pifferi S; Picci C; Gök C; Hoffmann D; Bali KK; Lampe A; Lapatsina L; Fleischer R; Smith ES; Bégay V; Moroni M; Estebanez L; Kühnemund J; Lewin GR

Nature neuroscience 2016

online lesen

Structural Characterization and Ligand/Inhibitor Identification Provide Functional Insights into the Mycobacterium tuberculosis Cytochrome P450 CYP126A1.

Chenge JT; Duyet LV; Swami S; McLean KJ; Kavanagh ME; Coyne AG; Rigby SE; Cheesman MR; Girvan HM; Levy CW; Rupp B; von Kries JP; Abell C; Leys D; Munro AW

The Journal of biological chemistry 2016

online lesen

Pharmacological restoration and therapeutic targeting of the B-cell phenotype in classical Hodgkin lymphoma.

Du J; Neuenschwander M; Yu Y; Däbritz JH; Neuendorff NR; Schleich K; Bittner A; Milanovic M; Beuster G; Radetzki S; Specker E; Reimann M; Schmitt CA

Blood 2016

online lesen

A Small-Molecule Antagonist of the β-Catenin/TCF4 Interaction Blocks the Self-Renewal of Cancer Stem Cells and Suppresses Tumorigenesis.

Fang L; Zhu Q; Neuenschwander M; Specker E; Wulf-Goldenberg A; Weis WI; von Kries JP; Birchmeier W

Cancer research 2015

online lesen

Substrate Hunting for the Myxobacterial CYP260A1 Revealed New 1α-Hydroxylated Products from C-19 Steroids.

Khatri Y; Ringle M; Lisurek M; von Kries JP; Zapp J; Bernhardt R

Chembiochem : a European journal of chemical biology 2015

online lesen

Selective inhibitors of the protein tyrosine phosphatase SHP2 block cellular motility and growth of cancer cells in vitro and in vivo.

Grosskopf S; Eckert C; Arkona C; Radetzki S; Böhm K; Heinemann U; Wolber G; von Kries JP; Birchmeier W; Rademann J

ChemMedChem 2015

online lesen

Design of a general-purpose European compound screening library for EU-OPENSCREEN.

Horvath D; Lisurek M; Rupp B; Kühne R; Specker E; von Kries J; Rognan D; Andersson CD; Almqvist F; Elofsson M; Enqvist PA; Gustavsson AL; Remez N; Frank R

ChemMedChem 2014

online lesen

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.

Voss FK; Ullrich F; Münch J; Lazarow K; Lutter D; Mah N; Andrade-Navarro MA; von Kries JP; Stauber T; Jentsch TJ

Science (New York, N.Y.) 2014

online lesen

Highly functionalized terpyridines as competitive inhibitors of AKAP-PKA interactions.

Schäfer G; Milić J; Eldahshan A; Götz F; Zühlke K; Schillinger C; Kreuchwig A; Elkins JM; Abdul Azeez KR; Oder A; Moutty MC; Masada N; Beerbaum M; Klussmann E

Angewandte Chemie (International ed. in English) 2013

online lesen

Small-molecule screening identifies modulators of aquaporin-2 trafficking.

Bogum J; Faust D; Zühlke K; Eichhorst J; Moutty MC; Furkert J; Eldahshan A; Neuenschwander M; von Kries JP; Wiesner B; Trimpert C; Deen PM; Klussmann E

Journal of the American Society of Nephrology : JASN 2013

online lesen

Identification of a new membrane-permeable inhibitor against inositol-1,4,5-trisphosphate-3-kinase A.

Schröder D; Rehbach C; Seyffarth C; Neuenschwander M; Kries JV; Windhorst S

Biochemical and biophysical research communications 2013

online lesen

Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.

Habermann K; Mirgorodskaya E; Gobom J; Lehmann V; Müller H; Blümlein K; Deery MJ; Czogiel I; Erdmann C; Ralser M; von Kries JP; Lange BM

Molecular and cellular biology 2012

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A novel tankyrase inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice.

Waaler J; Machon O; Tumova L; Dinh H; Korinek V; Wilson SR; Paulsen JE; Pedersen NM; Eide TJ; Machonova O; Gradl D; Voronkov A; von Kries JP; Krauss S

Cancer research 2012

online lesen

Identification of CYP106A2 as a regioselective allylic bacterial diterpene hydroxylase.

Bleif S; Hannemann F; Lisurek M; von Kries JP; Zapp J; Dietzen M; Antes I; Bernhardt R

Chembiochem : a European journal of chemical biology 2011

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Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth.

Waaler J; Machon O; von Kries JP; Wilson SR; Lundenes E; Wedlich D; Gradl D; Paulsen JE; Machonova O; Dembinski JL; Dinh H; Krauss S

Cancer research 2011

online lesen

Microtubule affinity regulating kinase activity in living neurons was examined by a genetically encoded fluorescence resonance energy transfer/fluorescence lifetime imaging-based biosensor: inhibitors with therapeutic potential.

Timm T; von Kries JP; Li X; Zempel H; Mandelkow E; Mandelkow EM

The Journal of biological chemistry 2011

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Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.

von Kleist L; Stahlschmidt W; Bulut H; Gromova K; Puchkov D; Robertson MJ; MacGregor KA; Tomilin N; Pechstein A; Chau N; Chircop M; Sakoff J; Haucke V

Cell 2011

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Bisubstrate specificity in histidine/tryptophan biosynthesis isomerase from Mycobacterium tuberculosis by active site metamorphosis.

Due AV; Kuper J; Geerlof A; von Kries JP; Wilmanns M

Proceedings of the National Academy of Sciences of the United States of America 2011

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Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes.

Christian F; Szaszák M; Friedl S; Drewianka S; Lorenz D; Goncalves A; Furkert J; Vargas C; Schmieder P; Götz F; Zühlke K; Moutty M; Göttert H; Klussmann E

The Journal of biological chemistry 2010

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The use of small molecule high-throughput screening to identify inhibitors of the proteinase 3-NB1 interaction.

Choi M; Eulenberg C; Rolle S; von Kries JP; Luft FC; Kettritz R

Clinical and experimental immunology 2010

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A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation.

Johnsen L; Weigel C; von Kries J; Møller M; Skarstad K

The Journal of antimicrobial chemotherapy 2010

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Identification of small-molecule scaffolds for p450 inhibitors.

von Kries JP; Warrier T; Podust LM

Current protocols in microbiology 2010

online lesen

Design of chemical libraries with potentially bioactive molecules applying a maximum common substructure concept.

Lisurek M; Rupp B; Wichard J; Neuenschwander M; von Kries JP; Frank R; Rademann J; Kühne R

Molecular diversity 2009

online lesen

Interaction of Mycobacterium tuberculosis CYP130 with heterocyclic arylamines.

Podust LM; Ouellet H; von Kries JP; de Montellano PR

The Journal of biological chemistry 2009

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Translocation Biosensors - Cellular System Integrators to Dissect CRM1-Dependent Nuclear Export by Chemicogenomics.

Fetz V; Knauer SK; Bier C; von Kries JP; Stauber RH

Sensors (Basel, Switzerland) 2009

online lesen

Indomethacin amides as a novel molecular scaffold for targeting Trypanosoma cruzi sterol 14alpha-demethylase.

Konkle ME; Hargrove TY; Kleshchenko YY; von Kries JP; Ridenour W; Uddin MJ; Caprioli RM; Marnett LJ; Nes WD; Villalta F; Waterman MR; Lepesheva GI

Journal of medicinal chemistry 2009

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Specific inhibitors of the protein tyrosine phosphatase Shp2 identified by high-throughput docking.

Hellmuth K; Grosskopf S; Lum CT; Würtele M; Röder N; von Kries JP; Rosario M; Rademann J; Birchmeier W

Proceedings of the National Academy of Sciences of the United States of America 2008

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X-ray structure of 4,4'-dihydroxybenzophenone mimicking sterol substrate in the active site of sterol 14alpha-demethylase (CYP51).

Eddine AN; von Kries JP; Podust MV; Warrier T; Kaufmann SH; Podust LM

The Journal of biological chemistry 2008

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Small-molecule scaffolds for CYP51 inhibitors identified by high-throughput screening and defined by X-ray crystallography.

Podust LM; von Kries JP; Eddine AN; Kim Y; Yermalitskaya LV; Kuehne R; Ouellet H; Warrier T; Alteköster M; Lee JS; Rademann J; Oschkinat H; Waterman MR

Antimicrobial agents and chemotherapy 2007

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The structure of human ADP-ribosylhydrolase 3 (ARH3) provides insights into the reversibility of protein ADP-ribosylation.

Mueller-Dieckmann C; Kernstock S; Lisurek M; von Kries JP; Haag F; Weiss MS; Koch-Nolte F

Proceedings of the National Academy of Sciences of the United States of America 2006

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Crystal structures and inhibitor identification for PTPN5, PTPRR and PTPN7: a family of human MAPK-specific protein tyrosine phosphatases.

Eswaran J; von Kries JP; Marsden B; Longman E; Debreczeni JE; Ugochukwu E; Turnbull A; Lee WH; Knapp S; Barr AJ

The Biochemical journal 2006

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Discovery of Mycobacterium tuberculosis protein tyrosine phosphatase A (MptpA) inhibitors based on natural products and a fragment-based approach.

Manger M; Scheck M; Prinz H; von Kries JP; Langer T; Saxena K; Schwalbe H; Fürstner A; Rademann J; Waldmann H

Chembiochem : a European journal of chemical biology 2005

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The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis.

Singh RK; Kefala G; Janowski R; Mueller-Dieckmann C; von Kries JP; Weiss MS

Journal of molecular biology 2004

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Hot spots in beta-catenin for interactions with LEF-1, conductin and APC.

von Kries JP; Winbeck G; Asbrand C; Schwarz-Romond T; Sochnikova N; Dell'Oro A; Behrens J; Birchmeier W

Nature structural biology 2000

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Functional interaction of beta-catenin with the transcription factor LEF-1.

Behrens J; von Kries JP; Kühl M; Bruhn L; Wedlich D; Grosschedl R; Birchmeier W

Nature 1996

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Nuclear matrix protein ARBP recognizes a novel DNA sequence motif with high affinity.

Buhrmester H; von Kries JP; Strätling WH

Biochemistry 1995

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Chicken MAR binding protein p120 is identical to human heterogeneous nuclear ribonucleoprotein (hnRNP) U.

von Kries JP; Buck F; Strätling WH

Nucleic acids research 1994

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Biochemical properties of attachment region binding protein ARBP.

von Kries JP; Rosorius O; Buhrmester H; Strätling WH

FEBS letters 1994

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A matrix/scaffold attachment region binding protein: identification, purification, and mode of binding.

von Kries JP; Buhrmester H; Strätling WH

Cell 1991

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A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.

von Kries JP; Phi-Van L; Diekmann S; Strätling WH

Nucleic acids research 1990

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The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes.