Dmytro Puchkov

Synaptotagmin 1-triggered lipid signaling facilitates coupling of exo- and endocytosis.

• Bolz S; Kaempf N; Puchkov D; Krauss M; Russo G; Soykan T; Schmied C; Lehmann M; Müller R; Schultz C; David Perrais; Maritzen T; Haucke V

Neuron 2023

Nutrient-regulated control of lysosome function by signaling lipid conversion

• Michael Ebner; Dmytro Puchkov; Orestes López-Ortega; Pathma Muthukottiappan; Yanwei Su; Christopher Schmied; Silke Zillmann; Iryna Nikonenko; Jochen Koddebusch; Gillian L. Dornan; Max T. Lucht; Vonda Koka; Wonyul Jang; Philipp Alexander Koch; Alexander Wallroth; Martin Lehmann; Britta Brügger; Mario Pende; Dominic Winter; Volker Haucke

Cell 2023

Phosphatidylinositol 3,5-bisphosphate facilitates axonal vesicle transport and presynapse assembly.

• Filiz Sila Rizalar; Max Thomas Lucht; Astrid G. Petzoldt; Kong S; Jiachen Sun; Vines JH; Narasimha Telugu; Sebastian Diecke; Thomas Kaas; Torsten Bullmann; Christopher Schmied; Löwe D; Jason S King; Cho W; Stefan Hallermann; Dmytro Puchkov; Stephan Sigrist; Volker Haucke

Science (New York, N.Y.) 2023

Adhesion energy controls lipid binding-mediated endocytosis

• Raluca Groza; Kita Valerie Schmidt; Paul Markus Müller; Paolo Ronchi; Claire Schlack-Leigers; Ursula Neu; Dmytro Puchkov; Rumiana Dimova; Claudia Matthäus; Justin Taraska; Thomas R. Weikl; Helge Ewers

Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM.

• Puchkov D; Müller PM; Lehmann M; Matthaeus C

Frontiers in cell and developmental biology 2023

Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse.

• Melina Overhoff; Frederik Tellkamp; Simon Hess; Marianna Tolve; Janine Tutas; Marcel Faerfers; Ickert L; Milad Mohammadi; De Bruyckere E; Kallergi E; Andrea Delle Vedove; Nikoletopoulou V; Brunhilde Wirth; Joerg Isensee; Tim Hucho; Dmytro Puchkov; Dirk Isbrandt; Krueger M; Peter Kloppenburg; Natalia Kononenko

The EMBO journal 2022

Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.

• Liu GT; Kochlamazashvili G; Dmytro Puchkov; Rainer Mueller; Carsten Schultz; Mackintosh AI; Vollweiter D; Volker Haucke; Tolga Soykan

The EMBO journal 2022

Inositol triphosphate-triggered calcium release from the endoplasmic reticulum induces lysosome biogenesis via TFEB/TFE3.

• mouhannad malek; Wawrzyniak AM; Michael Ebner; Puchkov D; Volker Haucke

The Journal of biological chemistry 2022

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function.

• Wonyul Jang; Dmytro Puchkov; Paula Samsó; YongTian Liang; Nadler-Holly M; Stephan Sigrist; Ulrich Kintscher; Fan Liu; kamel Mamchaoui; Vincent Mouly; Volker Haucke

Science (New York, N.Y.) 2022

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane.

• Claudia Matthaeus; Sochacki KA; Andrea Dickey; Dmytro Puchkov; Volker Haucke; Martin Lehmann; Justin Taraska

Nature communications 2022

Tight junctions in the blood–brain barrier promote edema formation and infarct size in stroke – Ambivalent effects of sealing proteins

• Winkler, L.; Blasig, R.; Breitkreuz-Korff, O.; Berndt, P.; Dithmer, S.; Helms, H.C.; Puchkov, D.; Devraj, K.; Kaya, M.; Qin, Z.; Liebner, S.; Wolburg, H.; Andjelkovic, A.V.; Rex, A.; Blasig, I.E.; Haseloff, R.F.

Journal of Cerebral Blood Flow and Metabolism 2020

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.

• Dmytro Puchkov

Neuron 2020

Novel amyloid-beta pathology C. elegans model reveals distinct neurons as seeds of pathogenicity.

• Gallrein C; Manuel Iburg; Michelberger T; Koçak A; Puchkov D; Fan Liu; Sara Maria Ayala Mariscal; Nayak T; Kaminski Schierle GS; Kirstein J

Progress in neurobiology 2020

Endocytic regulation of cellular ion homeostasis controls lysosome biogenesis.

• Tania López-Hernández; Dmytro Puchkov; Krause E; Tanja Maritzen; Volker Haucke

Nature cell biology 2020

Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration

• Weinert, S.; Gimber, N.; Deuschel, D.; Stuhlmann, T.; Puchkov, D.; Farsi, Z.; Ludwig, C.F.; Novarino, G.; López-Cayuqueo, K.I.; Planells-Cases, R.; Jentsch, T.J.

EMBO Journal 2020

Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway

• Wang, H.; Loerke, D.; Bruns, C.; Müller, R.; Koch, P.-A.; Puchkov, D.; Schultz, C.; Haucke, V.

Journal of Biological Chemistry 2020

The noncanonical small heat shock protein HSP-17 from Caenorhabditis elegans is a selective protein aggregase

• Iburg, M.; Puchkov, D.; Rosas-Brugada, I.U.; Bergemann, L.; Rieprecht, U.; Kirstein, J.

Journal of Biological Chemistry 2020

A Coincidence Detection Mechanism Controls PX-BAR Domain-Mediated Endocytic Membrane Remodeling via an Allosteric Structural Switch.

• Lo WT; Vujičić Žagar A; Gerth F; Lehmann M; Puchkov D; Krylova O; Freund C; Scapozza L; Vadas O; Haucke V

Developmental cell 2017

Intersectin associates with synapsin and regulates its nanoscale localization and function.

• Gerth F; Jäpel M; Pechstein A; Kochlamazashvili G; Lehmann M; Puchkov D; Onofri F; Benfenati F; Nikonenko AG; Fredrich K; Shupliakov O; Maritzen T; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2017

Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice by disrupting acrosome formation.

• Oberheide K; Puchkov D; Jentsch TJ

The Journal of biological chemistry 2017

Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly.

• Soykan T; Kaempf N; Sakaba T; Vollweiter D; Goerdeler F; Puchkov D; Kononenko NL; Haucke V

Neuron 2017

Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration.

• Kononenko NL; Claßen GA; Kuijpers M; Puchkov D; Maritzen T; Tempes A; Malik AR; Skalecka A; Bera S; Jaworski J; Haucke V

Nature communications 2017

Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1

• Rathjen, T.; Yan, X.; Kononenko, N.L.; Ku, M.-C.; Song, K.; Ferrarese, L.; Tarallo, V.; Puchkov, D.; Kochlamazashvili, G.; Brachs, S.; Varela, L.; Szigeti-Buck, K.; Yi, C.-X.; Schriever, S.C.; Tattikota, S.G.; Carlo, A.S.; Moroni, M.; Siemens, J.; Heuser, A.; Van Der Weyden, L.; Birkenfeld, A.L.; Niendorf, T.; Poulet, J.F.A.; Horvath, T.L.; Tschöp, M.H.; Heinig, M.; Trajkovski, M.; Haucke, V.; Poy, M.N.

Nature Neuroscience 2017

Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission

• Seong Joo Koo; Gaga Kochlamazashvili; Benjamin Rost; Dmytro Puchkov; Niclas Gimber; Martin Lehmann; Georgi Tadeus; Jan Schmoranzer; Christian Rosenmund; Volker Haucke; Tanja Maritzen

Neuron 2015

Overlapping functions of stonin 2 and SV2 in sorting of the calcium sensor synaptotagmin 1 to synaptic vesicles

• Natalie Kaempf; Gaga Kochlamazashvili; Dmytro Puchkov; Tanja Maritzen; Sandra M. Bajjalieh; Natalia L. Kononenko; Volker Haucke

Proc Natl Acad Sci USA 2015

Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain

• Martin Lehmann; Benjamin Gottschalk; Dmytro Puchkov; Peter Schmieder; Sergej Schwagerus; Christian P. R. Hackenberger; Volker Haucke; Jan Schmoranzer

Angew. Chem. 2015

Muscular Dystrophy Mutations Impair the Nuclear Envelope Emerin Self-assembly Properties

• Isaline Herrada; Camille Samson; Christophe Velours; Louis Renault; Cecilia Östlund; Pierre Chervy; Dmytro Puchkov; Howard J Worman; Brigitte Buendia; Sophie Zinn-Justin

ACS Chem. Biol. 2015

Greasing the synaptic vesicle cycle by membrane lipids

• Dmytro Puchkov; Volker Haucke

Trends in Cell Biology 2013

Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate

• York Posor; Marielle Eichhorn-Gruenig; Dmytro Puchkov; Johannes Schöneberg; Alexander Ullrich; André Lampe; Rainer Müller; Sirus Zarbakhsh; Federico Gulluni; Emilio Hirsch; Michael Krauss; Carsten Schultz; Jan Schmoranzer; Frank Noé; Volker Haucke

Nature 2013

Compromised fidelity of endocytic synaptic vesicle protein sorting in the absence of stonin 2.

• Kononenko NL; Diril MK; Puchkov D; Kintscher M; Koo SJ; Pfuhl G; Winter Y; Wienisch M; Klingauf J; Breustedt J; Schmitz D; Maritzen T; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2013

The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms

• Shetty, A.; Sytnyk, V.; Leshchyns'ka, I.; Puchkov, D.; Haucke, V.; Schachner, M.

Journal of Neuroscience 2013

NCAM/spectrin complex disassembly results in PSD perforation and postsynaptic endocytic zone formation

• Puchkov, D.; Leshchyns'Ka, I.; Nikonenko, A.G.; Schachner, M.; Sytnyk, V.

Cerebral Cortex 2011

AP180 and CALM

• Seong Joo Koo; Dmytro Puchkov; Volker Haucke

Cellular Logistics 2011

Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition

• Lisa von Kleist; Wiebke Stahlschmidt; Haydar Bulut; Kira Gromova; Dmytro Puchkov; Mark J. Robertson; Kylie A. MacGregor; Nikolay Tomilin; Arndt Pechstein; Ngoc Chau; Megan Chircop; Jennette Sakoff; Jens Peter von Kries; Wolfram Saenger; Hans-Georg Kräusslich; Oleg Shupliakov; Phillip J. Robinson; Adam McCluskey; Volker Haucke

Cell 2011

SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses.

• Koo SJ; Markovic S; Puchkov D; Mahrenholz CC; Beceren-Braun F; Maritzen T; Dernedde J; Volkmer R; Oschkinat H; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2011