Dmytro Puchkov

Canonical and non-canonical integrin-based adhesions dynamically interconvert.

• Lukas F; Matthaeus C; López-Hernández T; Lahmann I; Schultz N; Lehmann M; Puchkov D; Pielage J; Haucke V; Maritzen T

Nature communications 2024

Adhesion energy controls lipid binding-mediated endocytosis

• Groza, R.; Schmidt, K.V.; Müller, P.M.; Ronchi, P.; Schlack-Leigers, C.; Neu, U.; Puchkov, D.; Dimova, R.; Matthaeus, C.; Taraska, J.; Weikl, T.R.; Ewers, H.

Nature Communications 2024

Human iPSC-Derived Neurons with Reliable Synapses and Large Presynaptic Action Potentials

• Bullmann, T.; Kaas, T.; Ritzau-Jost, A.; Wöhner, A.; Kirmann, T.; Rizalar, F.S.; Holzer, M.; Nerlich, J.; Puchkov, D.; Geis, C.; Eilers, J.; Kittel, R.J.; Arendt, T.; Haucke, V.; Hallermann, S.

Journal of Neuroscience 2024

Sociality shapes mitochondrial adaptations supporting hypoxia tolerance

• Rossi, A.; Ruwolt, M.; Kakouri, P.; Kosten, T.; Kunz, S.; Puchkov, D.; Reznick, J.; Purfürst, B.; Omerbašić, D.; Aranda, D.M.; Carai, G.; Mastrobuoni, G.; Hart, D.W.; Carraro, M.; Tommasin, L.; Bennett, N.C.; Bégay, V.; Faelber, K.; Daumke, O.; Bernardi, P.; Park, T.J.; Kempa, S.; Liu, F.; Lewin, G.R.

Biorxiv 2024

Integrative Molecular Dynamics Simulations Untangle Cross-Linking Data to Unveil Mitochondrial Protein Distributions

• Schuhmann, F.; Akkaya, K.C.; Puchkov, D.; Lehmann, M.; Liu, F.; Pezeshkian, W.

Biorxiv 2024

ARF1 compartments direct cargo flow via maturation into recycling endosomes

• Stockhammer, A.; Adarska, P.; Natalia, V.; Heuhsen, A.; Klemt, A.; Bregu, G.; Harel, S.; Rodilla-Ramirez, C.; Spalt, C.; Özsoy, E.; Leupold, P.; Grindel, A.; Fox, E.; Mejedo, J.O.; Zehtabian, A.; Ewers, H.; Puchkov, D.; Haucke, V.; Bottanelli, F.

Nature Cell Biology 2024

LRRC8/VRAC volume-regulated anion channels are crucial for hearing

• Knecht, D.A.; Zeziulia, M.; Bhavsar, M.B.; Puchkov, D.; Maier, H.; Jentsch, T.J.

Journal of Biological Chemistry 2024

Synaptotagmin 1-triggered lipid signaling facilitates coupling of exo- and endocytosis.

• Bolz S; Kaempf N; Puchkov D; Krauss M; Russo G; Soykan T; Schmied C; Lehmann M; Müller R; Schultz C; Perrais D; Maritzen T; Haucke V

Neuron 2023

Nutrient-regulated control of lysosome function by signaling lipid conversion

• Michael Ebner; Dmytro Puchkov; Orestes López-Ortega; Pathma Muthukottiappan; Yanwei Su; Christopher Schmied; Silke Zillmann; Iryna Nikonenko; Jochen Koddebusch; Gillian L. Dornan; Max T. Lucht; Vonda Koka; Wonyul Jang; Philipp Alexander Koch; Alexander Wallroth; Martin Lehmann; Britta Brügger; Mario Pende; Dominic Winter; Volker Haucke

Cell 2023

Phosphatidylinositol 3,5-bisphosphate facilitates axonal vesicle transport and presynapse assembly.

• Rizalar FS; Lucht MT; Petzoldt A; Kong S; Sun J; Vines JH; Telugu NS; Diecke S; Kaas T; Bullmann T; Schmied C; Löwe D; King JS; Cho W; Hallermann S; Puchkov D; Sigrist SJ; Haucke V

Science (New York, N.Y.) 2023

Adhesion energy controls lipid binding-mediated endocytosis

• Raluca Groza; Kita Valerie Schmidt; Paul Markus Müller; Paolo Ronchi; Claire Schlack-Leigers; Ursula Neu; Dmytro Puchkov; Rumiana Dimova; Claudia Matthäus; Justin Taraska; Thomas R. Weikl; Helge Ewers

Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM.

• Puchkov D; Müller PM; Lehmann M; Matthaeus C

Frontiers in cell and developmental biology 2023

Erratum: Synaptotagmin 1-triggered lipid signaling facilitates coupling of exo- and endocytosis (Neuron (2023) 111(23) (3765–3774.e7), (S0896627323006281), (10.1016/j.neuron.2023.08.016))

• Bolz, S.; Kaempf, N.; Puchkov, D.; Krauss, M.; Russo, G.; Soykan, T.; Schmied, C.; Lehmann, M.; Müuller, R.; Schultz, C.; Perrais, D.; Maritzen, T.; Haucke, V.

Neuron 2023

ARF1 compartments direct cargo flow via maturation into recycling endosomes

• Stockhammer, A.; Adarska, P.; Natalia, V.; Heuhsen, A.; Klemt, A.; Bregu, G.; Harel, S.; Rodilla-Ramirez, C.; Spalt, C.; Özsoy, E.; Leupold, P.; Grindel, A.; Fox, E.; Mejedo, J.O.; Zehtabian, A.; Ewers, H.; Puchkov, D.; Haucke, V.; Bottanelli, F.

Biorxiv 2023

Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse.

• Overhoff M; Tellkamp F; Hess S; Tolve M; Tutas J; Faerfers M; Ickert L; Mohammadi M; De Bruyckere E; Kallergi E; Delle Vedove A; Nikoletopoulou V; Wirth B; Isensee J; Hucho T; Puchkov D; Isbrandt D; Krueger M; Kloppenburg P; Kononenko NL

The EMBO journal 2022

Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.

• Liu GT; Kochlamazashvili G; Puchkov D; Müller R; Schultz C; Mackintosh AI; Vollweiter D; Haucke V; Soykan T

The EMBO journal 2022

Inositol triphosphate-triggered calcium release from the endoplasmic reticulum induces lysosome biogenesis via TFEB/TFE3.

• Malek M; Wawrzyniak AM; Ebner M; Puchkov D; Haucke V

The Journal of biological chemistry 2022

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function.

• Jang W; Puchkov D; Samsó P; Liang Y; Nadler-Holly M; Sigrist SJ; Kintscher U; Liu F; Mamchaoui K; Mouly V; Haucke V

Science (New York, N.Y.) 2022

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane.

• Matthaeus C; Sochacki KA; Dickey AM; Puchkov D; Haucke V; Lehmann M; Taraska JW

Nature communications 2022

Autophagy regulates neuronal excitability by controlling cAMP/Protein Kinase A signaling

• Overhoff, M.; Tellkamp, F.; Hess, S.; Tutas, J.; Tolve, M.; Faerfers, M.; Ickert, L.; Mohammadi, M.; de Bruyckere, E.; Kallergi, E.; Dell Vedove, A.; Nikoletopoulou, V.; Wirth, B.; Isensee, J.; Hucho, T.; Puchkov, D.; Isbrandt, D.; Krüger, M.; Kloppenburg, P.; Kononenko, N.L.

Biorxiv 2022

The molecular organization of flat and curved caveolae indicates bendable structural units at the plasma membrane

• Matthaeus, C.; Sochacki, K.A.; Dickey, A.; Puchkov, D.; Haucke, V.; Lehmann, M.; Taraska, J.W.

Biorxiv 2022

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.

• Dmytro Puchkov

Neuron 2020

Novel amyloid-beta pathology C. elegans model reveals distinct neurons as seeds of pathogenicity.

• Gallrein C; Iburg M; Michelberger T; Koçak A; Puchkov D; Liu F; Ayala Mariscal SM; Nayak T; Kaminski Schierle GS; Kirstein J

Progress in neurobiology 2020

Endocytic regulation of cellular ion homeostasis controls lysosome biogenesis.

• López-Hernández T; Puchkov D; Krause E; Maritzen T; Haucke V

Nature cell biology 2020

Neuronal autophagy regulates presynaptic neurotransmission by controlling the axonal endoplasmic reticulum

• Kuijpers, M.; Kochlamazashvili, G.; Stumpf, A.; Puchkov, D.; Lucht, M.T.; Krause, E.; Schmitz, D.; Haucke, V.

Biorxiv 2020

Presynaptic precursor vesicles originate from the trans-Golgi network, promoted by the small GTPase RAB2

• Götz, T.W.B.; Puchkov, D.; Lützkendorf, J.; Nikonenko, A.G.; Quentin, C.; Lehmann, M.; Sigrist, S.J.; Petzoldt, A.G.

Biorxiv 2020

Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration

• Weinert, S.; Gimber, N.; Deuschel, D.; Stuhlmann, T.; Puchkov, D.; Farsi, Z.; Ludwig, C.F.; Novarino, G.; López-Cayuqueo, K.I.; Planells-Cases, R.; Jentsch, T.J.

EMBO Journal 2020

Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway

• Wang, H.; Loerke, D.; Bruns, C.; Müller, R.; Koch, P.-A.; Puchkov, D.; Schultz, C.; Haucke, V.

Journal of Biological Chemistry 2020

The noncanonical small heat shock protein HSP-17 from Caenorhabditis elegans is a selective protein aggregase

• Iburg, M.; Puchkov, D.; Rosas-Brugada, I.U.; Bergemann, L.; Rieprecht, U.; Kirstein, J.

Journal of Biological Chemistry 2020

A Coincidence Detection Mechanism Controls PX-BAR Domain-Mediated Endocytic Membrane Remodeling via an Allosteric Structural Switch.

• Lo WT; Vujičić Žagar A; Gerth F; Lehmann M; Puchkov D; Krylova O; Freund C; Scapozza L; Vadas O; Haucke V

Developmental cell 2017

Intersectin associates with synapsin and regulates its nanoscale localization and function.

• Gerth F; Jäpel M; Pechstein A; Kochlamazashvili G; Lehmann M; Puchkov D; Onofri F; Benfenati F; Nikonenko AG; Fredrich K; Shupliakov O; Maritzen T; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2017

Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice by disrupting acrosome formation.

• Oberheide K; Puchkov D; Jentsch TJ

The Journal of biological chemistry 2017

Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly.

• Soykan T; Kaempf N; Sakaba T; Vollweiter D; Goerdeler F; Puchkov D; Kononenko NL; Haucke V

Neuron 2017

Erratum: Intersectin associates with synapsin and regulates its nanoscale localization and function (Proceedings of the National Academy of Sciences of the United States of America (2017) 114 (12057-12062) DOI: 10.1073/pnas.1715341114)

• Gerth, F.; Jäpel, M.; Pechstein, A.; Kochlamazashvili, G.; Lehmann, M.; Puchkov, D.; Onofri, F.; Benfenati, F.; Nikonenko, A.G.; Fredrich, K.; Shupliakov, O.; Maritzen, T.; Freund, C.; Haucke, V.

Proceedings of the National Academy of Sciences of the United States of America 2017

Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration.

• Kononenko NL; Claßen GA; Kuijpers M; Puchkov D; Maritzen T; Tempes A; Malik AR; Skalecka A; Bera S; Jaworski J; Haucke V

Nature communications 2017

Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1

• Rathjen, T.; Yan, X.; Kononenko, N.L.; Ku, M.-C.; Song, K.; Ferrarese, L.; Tarallo, V.; Puchkov, D.; Kochlamazashvili, G.; Brachs, S.; Varela, L.; Szigeti-Buck, K.; Yi, C.-X.; Schriever, S.C.; Tattikota, S.G.; Carlo, A.S.; Moroni, M.; Siemens, J.; Heuser, A.; Van Der Weyden, L.; Birkenfeld, A.L.; Niendorf, T.; Poulet, J.F.A.; Horvath, T.L.; Tschöp, M.H.; Heinig, M.; Trajkovski, M.; Haucke, V.; Poy, M.N.

Nature Neuroscience 2017

Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission

• Seong Joo Koo; Gaga Kochlamazashvili; Benjamin Rost; Dmytro Puchkov; Niclas Gimber; Martin Lehmann; Georgi Tadeus; Jan Schmoranzer; Christian Rosenmund; Volker Haucke; Tanja Maritzen

Neuron 2015

Overlapping functions of stonin 2 and SV2 in sorting of the calcium sensor synaptotagmin 1 to synaptic vesicles

• Natalie Kaempf; Gaga Kochlamazashvili; Dmytro Puchkov; Tanja Maritzen; Sandra M. Bajjalieh; Natalia L. Kononenko; Volker Haucke

Proc Natl Acad Sci USA 2015

Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain

• Martin Lehmann; Benjamin Gottschalk; Dmytro Puchkov; Peter Schmieder; Sergej Schwagerus; Christian P. R. Hackenberger; Volker Haucke; Jan Schmoranzer

Angew. Chem. 2015

Muscular Dystrophy Mutations Impair the Nuclear Envelope Emerin Self-assembly Properties

• Isaline Herrada; Camille Samson; Christophe Velours; Louis Renault; Cecilia Östlund; Pierre Chervy; Dmytro Puchkov; Howard J Worman; Brigitte Buendia; Sophie Zinn-Justin

ACS Chem. Biol. 2015

Greasing the synaptic vesicle cycle by membrane lipids

• Dmytro Puchkov; Volker Haucke

Trends in Cell Biology 2013

Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate

• York Posor; Marielle Eichhorn-Gruenig; Dmytro Puchkov; Johannes Schöneberg; Alexander Ullrich; André Lampe; Rainer Müller; Sirus Zarbakhsh; Federico Gulluni; Emilio Hirsch; Michael Krauss; Carsten Schultz; Jan Schmoranzer; Frank Noé; Volker Haucke

Nature 2013

Compromised fidelity of endocytic synaptic vesicle protein sorting in the absence of stonin 2.

• Kononenko NL; Diril MK; Puchkov D; Kintscher M; Koo SJ; Pfuhl G; Winter Y; Wienisch M; Klingauf J; Breustedt J; Schmitz D; Maritzen T; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2013

The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms

• Shetty, A.; Sytnyk, V.; Leshchyns'ka, I.; Puchkov, D.; Haucke, V.; Schachner, M.

Journal of Neuroscience 2013

NCAM/spectrin complex disassembly results in PSD perforation and postsynaptic endocytic zone formation

• Puchkov, D.; Leshchyns'Ka, I.; Nikonenko, A.G.; Schachner, M.; Sytnyk, V.

Cerebral Cortex 2011

AP180 and CALM

• Seong Joo Koo; Dmytro Puchkov; Volker Haucke

Cellular Logistics 2011

Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition

• Lisa von Kleist; Wiebke Stahlschmidt; Haydar Bulut; Kira Gromova; Dmytro Puchkov; Mark J. Robertson; Kylie A. MacGregor; Nikolay Tomilin; Arndt Pechstein; Ngoc Chau; Megan Chircop; Jennette Sakoff; Jens Peter von Kries; Wolfram Saenger; Hans-Georg Kräusslich; Oleg Shupliakov; Phillip J. Robinson; Adam McCluskey; Volker Haucke

Cell 2011

SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses.

• Koo SJ; Markovic S; Puchkov D; Mahrenholz CC; Beceren-Braun F; Maritzen T; Dernedde J; Volkmer R; Oschkinat H; Haucke V

Proceedings of the National Academy of Sciences of the United States of America 2011